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Western blot analysis of Paralemmin3 in MCF7 cell lysate with Paralemmin3 antibody at 1 μg/mL.
Immunocytochemistry of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 5 μg/mL.
Immunofluorescence of Paralemmin 3 in MCF7 cells with Paralemmin 3 antibody at 20 μg/mL.
Western blot analysis of Paralemmin3 in MCF7 cell lysate with Paralemmin3 antibody at 1 μg/mL.
Trial Size

Paralemmin3 Antibody

CATALOG NUMBER: 6449

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (79%)
Immunogen:
Paralemmin3 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human Paralemmin3.
The immunogen is located within amino acids 50 - 100 of Paralemmin3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Paralemmin3 antibody can be used for detection of Paralemmin3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of Paralemmin3 are known to exist; this antibody will only detect the longer isoform
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Positive Control 2:
Cat. No. 17-012 - MCF7 Cell Slide
Purification:
Paralemmin3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Paralemmin3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Paralemmin3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PALM3
Additional Names:
Paralemmin3 Antibody: Paralemmin-3
Protein Accession Number:
NP_001138500
Protein Gi Number:
222537747
Ncbi Gene Id Number:
342979
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Paralemmin3 Antibody: Paralemmin3 is a recently identified protein that associates with the single immunoglobulin IL-1 receptor-related molecule (SIGIRR), a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Silencing of Paralemmin3 expression by RNAi inhibited the release of inflammatory cytokines in A549 cells after stimulation with lipopolysaccharide (LPS), suggesting Paralemmin3 may function as an adaptor in the LPS-TLR4 signaling pathway.
Background Reference 1:
Chen X, Wu X, Zhao Y, et al. A novel binding protein of single immunoglobulin IL-1 receptor-related molecule: Paralemmin-3. Biochem. Biophys. Res. Commun. 2011; 404:1029-33
Background Reference 2:
Wald D, Qin J, Zhao Z, et al. SIGIRR, a negative regulator of Toll-like receptor-interleukin 1 receptor signaling. Nat. Immunol. 2003; 4:920-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6449

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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