Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of AFAP1L1 in A549 cell lysate with AFAP1L1 antibody at 1 μg/mL.
Immunocytochemistry of AFAP1L in A549 cells with AFAP1L antibody at 5 μg/mL.
Immunofluorescence of AFAP1L in A549 cells with AFAP1L antibody at 20 μg/mL.
Western blot analysis of AFAP1L1 in A549 cell lysate with AFAP1L1 antibody at 1 μg/mL.
Trial Size

AFAP1L Antibody

CATALOG NUMBER: 6559

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (79%)
Immunogen:
AFAP1L1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human AFAP1L1.
The immunogen is located within amino acids 70 - 120 of AFAP1L.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
AFAP1L1 antibody can be used for detection of AFAP1L1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of AFAP1L1 are known to exist. This antibody is predicted to not cross-react with other AFAP family members.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 17-003 - A549 Cell Slide
Purification:
AFAP1L Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AFAP1L Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AFAP1L antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
AFAP1L1
Additional Names:
AFAP1L Antibody: Actin filament-associated protein 1-like 1, AFAP1-like protein 1
Protein Accession Number:
NP_689619
Protein Gi Number:
55742689
Ncbi Gene Id Number:
134265
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
AFAP1L Antibody: The actin filament-associated protein 1 (AFAP1)-like 1 protein is one of three members in the AFAP1 family of adaptor proteins. Like AFAP1, AFAP1L1 is an actin binding protein that has a role in actin cytoskeleton arrangement, but AFAP1L1 is thought to have a unique function distinct from AFAP1. AFAP1L1 colocalizes with cortactin and localizes to invadosomes, highly dynamic, actin-rich adhesion structures harboring metalloproteases, suggesting that AFAP1L1 affects invadosome formation (1, 4). Recent reports suggest that AFAP1L1 can be used as a prognostic marker for spindle cell sarcomas.
Background Reference 1:
Snyder BN, Cho Y, Qian Y, et al. AFAP1L1 is a novel adaptor protein of the AFAP family that interacts with cortactin and localizes to invadosomes. Eur. J. Cell Biol. 2011; 90:376-89
Background Reference 2:
Flynn DC, Leu TH, Reynolds AB, et al. Identification and sequence analysis of cDNAs encoding a 110-kilodalton actin filament-associated pp60src substrate. Mol. Cell. Biol. 1993;13:7892-900.
Background Reference 3:
Baisden JM, Gatesman AS, Cherezova L, et al. The intrinsic ability of AFAP-110 to alter actin filament integrity is linked with its ability to also activate cellular tyrosine kinases. Oncogene 2001; 20:6607-16.
Background Reference 4:
Saltel F, Daubon T, Juin A, et al. Invadosomes: intriguing structures with promise. Eur. J. Cell Biol. 2011; 90:100-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6559

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369