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Western blot analysis of PRICKLE2 in A-20 lysate with PRICKLE2 antibody at 1 μg/mL.
Immunofluorescence of PRICKLE2 in rat brain cells with PRICKLE2 antibody at 20 μg/mL.
Western blot analysis of PRICKLE2 in A-20 lysate with PRICKLE2 antibody at 1 μg/mL.
Trial Size

PRICKLE2 Antibody

CATALOG NUMBER: 6563

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PRICKLE2 antibody was raised against a 15 amino acid synthetic peptide near the center of human PRICKLE2.
The immunogen is located within amino acids 420 - 470 of PRICKLE2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
PRICKLE2 antibody can be used for detection of PRICKLE2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Specificity:
Two isoforms of PRICKLE2 are known to exist. PRICKLE2 antibody is predicted to not cross-react with other PRICKLE protein family members.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Purification:
PRICKLE2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PRICKLE2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PRICKLE2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRICKLE2
Additional Names:
PRICKLE2 Antibody: EPM5, Prickle-like protein 2
Protein Accession Number:
NP_942559
Protein Gi Number:
38524620
Ncbi Gene Id Number:
166336
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRICKLE2 Antibody: PRICKLE2, also known as Pk2 or EPM5, is a member of a highly conserved protein family that function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity. Both PRICKLE2 and the related protein PRICKLE1 are expressed in postmitotic neurons and promote neurite outgrowth, and both proteins promote neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. PRICKLE2 localizes to the postsynaptic density and interacts with PSD-95 and NMDA receptors. Defects in the gene encoding PRICKLE2 are associated with autosomal recessive progressive myoclonic epilepsy.
Background Reference 1:
Katoh M and Katoh M. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle. Int. J. Mol. Med. 2003; 11:249-56.
Background Reference 2:
Veeman MT, Slusarski DC, Kaykas A, et al. Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Curr. Biol. 2003; 13:680-5.
Background Reference 3:
Okuda H, Miyata S, Mori Y, et al. Mouse Prickle1 and Prickle2 are expressed in postmitotic neurons and promote neurite outgrowth. FEBS Lett. 2007; 581:4754-60
Background Reference 4:
Fujimura L, Watanabe-Takano H, Sato Y, et al. Prickle promotes neurite outgrowth via the Dishevelled dependent pathway in C1300 cells. Neurosci. Lett. 2009; 467:6-10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6563

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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