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Western blot analysis of LEMD1 in A20 cell lysate with LEMD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of LEMD1 in A20 cell lysate with LEMD1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

LEMD1 Antibody

CATALOG NUMBER: 6599

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
LEMD1 antibody was raised against a 19 amino acid synthetic peptide near the center of human LEMD1.
The immunogen is located within amino acids 90 - 140 of LEMD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
LEMD1 antibody can be used for detection of LEMD1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificity:
At least six isoforms of LEMD1 are known to exist; this antibody will detect the longest isoform. LEMD1 antibody is predicted to not cross-react with LEMD2 and LEMD3.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Purification:
LEMD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LEMD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LEMD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LEMD1
Additional Names:
LEMD1 Antibody: CT50, LEMP-1, LEM domain-containing protein 1, LEM domain protein 1, CT50
Protein Accession Number:
Q68G75
Protein Gi Number:
312261248
Ncbi Gene Id Number:
93273
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LEMD1 Antibody: LEMD1 (LEM domain-containing1) is a member of cancer-testis gene family. LEMD1 is normally expressed only in testis. Six alternatively spliced forms of LEMD1 transcripts in normal testis have been identified, but only one of the six was expressed in colorectal cancers. LEMD1 may represent a promising target antigen for immunotherapy of colorectal cancers. In addition, LEMD1 and SPATA19 are putative cancer biomarkers and promising targets for active immunotherapy.
Background Reference 1:
Yuki D, Lin YM, Fujii Y, et al. Isolation of LEM domain-containing 1, a novel testis-specific gene expressed in colorectal cancers. Oncol. Rep. 2004; 12:275-80
Background Reference 2:
Ghafouri-Fard S, Ousati Ashtiani Z, Sabah Golian B, et al. Expression of two testis-specific genes, SPATA19 and LEMD1, in prostate cancer. Arch. Med. Res. 2010; 41:195-200

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6599

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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