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Western blot analysis of SPATA18 in rat lung tissue lysate with SPATA18 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Western blot analysis of SPATA18 in rat lung tissue lysate with SPATA18 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Trial Size

SPATA18 Antibody

CATALOG NUMBER: 6615

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
SPATA18 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human SPATA18.
The immunogen is located within amino acids 90 - 140 of SPATA18.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
SPATA18 antibody can be used for detection of SPATA18 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of SPATA18 are known to exist; this antibody will detect the two longest isoforms. SPATA18 antibody is predicted to not cross-react with other SPATA family members.
Positive Control 1:
Cat. No. 1462 - Rat Lung Tissue Lysate
Purification:
SPATA18 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
SPATA18 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SPATA18 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
SPATA18
Additional Names:
SPATA18 Antibody: Mieap, SPETEX1, MIEAP, Mitochondria-eating protein, Spermatogenesis-associated protein 18
Protein Accession Number:
NP_660306
Protein Gi Number:
21687119
Ncbi Gene Id Number:
132671
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
SPATA18 Antibody: SPATA18, also known as SPETEX-1, was initially identified as a cytoplasmic protein of elongate spermatids that is thought to be involved in differentiation process of elongate spermatids to mature spermatozoa. Recent experiments have shown that SPATA18 is a transcriptional target of p53 and p63. It has also been suggested to play a role in mitochondria quality control by inducing intramitochondrial lysosome-like organella that eliminate oxidized mitochondrial proteins.
Background Reference 1:
Iida H, Ichinose J, Kaneko T, et al. Complementary DNA cloning of rat spetex-1, a spermatid-expressing gene-1, encoding a 63 kDa cytoplasmic protein of elongate spermatids. Mol. Reprod. Dev. 2004; 68:385-93.
Background Reference 2:
Bornstein C, Brosh R, Molchadsky A, et al. SPATA18, a spermatogenesis-associated gene, is a novel transcriptional target of p53 and p63. Mol. Cell Biol. 2011; 31:1679-89
Background Reference 3:
Miyamoto Y, Kitamura N, Nakamura Y, et al. Possible existence of lysosome-like organella within mitochondria and its role in mitochondrial quality control. PLoS One 2011; 6:e16054.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6615

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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