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Western blot analysis of TSHZ1 in A-20 cell lysate with TSHZ1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of TSHZ1 in A20 cells with TSHZ1 antibody at 2.5 μg/mL.
Immunofluorescence of TSHZ1 in A20 cells with TSHZ1 antibody at 20 μg/mL.
Western blot analysis of TSHZ1 in A-20 cell lysate with TSHZ1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

TSHZ1 Antibody

CATALOG NUMBER: 6755

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TSHZ1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human TSHZ1.
The immunogen is located within amino acids 70 - 120 of TSHZ1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
TSHZ1 antibody can be used for detection of TSHZ1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 17-208 - A-20 Cell Slide
Purification:
TSHZ1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TSHZ1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TSHZ1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TSHZ1
Additional Names:
TSHZ1 Antibody: CAA, TSH1, NY-CO-33, SDCCAG33, Teashirt homolog 1, Antigen NY-CO-33
Protein Accession Number:
NP_005777
Protein Gi Number:
38201677
Ncbi Gene Id Number:
10194
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TSHZ1 Antibody: The Teashirt zinc finger homeobox (TSHZ) family comprise a family of evolutionarily conserved transcription factors that, in Drosophila, are active in specific body parts for patterning, but whose function in vertebrates is less clear. TSHZ1 has been found to be required for axial skeleton, soft palate and middle ear development in mice and may be involved in a common pathway with the Hox genes. Both TSHZ1 and the related protein TSHZ3 have been found to interact with FE65, an adapter protein that binds to the amyloid protein precursor (APP) in neurons. Together with SET, a component of the inhibitor of acetyl transferase, and histone deacetylases, these proteins formed a gene-silencing complex whose target includes caspase-4.
Background Reference 1:
Caubit X, Core N, Boned A, et al. Vertebrate orthologues of the Drosophila region-specific patterning gene teashirt. Mech. Dev. 2000; 91:445-8.
Background Reference 2:
Santos JS, Fonseca NA, Vieira CP, et al. Phylogeny of the Teashirt-related zinc finger (tshz) gene family and analysis of the developmental expression of tshz2 and tshz3 in the zebrafish. Dev. Dyn. 2010; 239:1010-8.
Background Reference 3:
Core N, Caubit X, Metchat A, et al. Tshz1 is required for axial skeleton, soft palate and middle ear development in mice. Dev. Biol. 2007; 308:407-20
Background Reference 4:
Kajiwara Y, Akram A, Katsel P, et al. FE65 binds teashirt, inhibiting expression of the primate-specific caspase-4. PLoS One 2009; 4:e5071.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6755

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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