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Western blot analysis of YPEL2 in HeLa cell lysate with YPEL2 antibody at 1 μg/mL.
Immunocytochemistry of YPEL2 in HeLa cells with YPEL2 antibody at 2.5 μg/mL.
Immunofluorescence of YPEL2 in HeLa cells with YPEL2 antibody at 5 μg/mL.
Western blot analysis of YPEL2 in HeLa cell lysate with YPEL2 antibody at 1 μg/mL.
Trial Size

YPEL2 Antibody

CATALOG NUMBER: 6773

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
YPEL2 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human YPEL2.
The immunogen is located within amino acids 30 - 80 of YPEL2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
YPEL2 antibody can be used for detection of YPEL2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Purification:
YPEL2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
YPEL2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
YPEL2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
YPEL2
Additional Names:
YPEL2 Antibody: FKSG4, Protein yippee-like 2
Protein Accession Number:
EAW94407
Protein Gi Number:
53292625
Ncbi Gene Id Number:
388403
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
YPEL2 Antibody: YPEL2 (yippee-like 2) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL2 might be an important factor during the development and malignant transformation of tissues, most notably pancreatic and breast tumors.
Background Reference 1:
Hosono K, Sasaki T, Minoshima S, et al. Identification and characterization of a novel gene family YPEL in a wide spectrum of eukaryotic species. Gene 2004; 340: 31-43.
Background Reference 2:
Couch FJ, Wang X, Bamlet WR, et al. Association of mitotic regulation pathway polymorphisms with pancreatic cancer risk and outcome. Cancer Epidemiol. Biomarkers Prev. 2010; 19:251-7
Background Reference 3:
Olson JE, Wang X, Pankratz VS, et al. Centrosome-related genes, genetic variation, and risk of breast cancer. Breast Cancer Res. Treat. 2011; 125:221-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6773

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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