Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of YPEL3 in A-20 cell lysate with YPEL3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Immunocytochemistry of YPEL3 in A20 cells with YPEL3 antibody at 2.5 μg/mL.
Immunofluorescence of YPEL3 in A20 cells with YPEL3 antibody at 5 μg/mL.
Western blot analysis of YPEL3 in A-20 cell lysate with YPEL3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide
Trial Size

YPEL3 Antibody

CATALOG NUMBER: 6775

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
YPEL3 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human YPEL3.
The immunogen is located within amino acids 20 - 70 of YPEL3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
YPEL3 antibody can be used for detection of YPEL3 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 17-208 - A-20 Cell Slide
Purification:
YPEL3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
YPEL3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
YPEL3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
YPEL3
Additional Names:
YPEL3 Antibody: Protein yippee-like 3
Protein Accession Number:
NP_113665
Protein Gi Number:
117956405
Ncbi Gene Id Number:
83719
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
YPEL3 Antibody: YPEL3 (yippee-like 3) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL3 is a p53-regulated gene whose expression is induced by DNA damage and in turn induces cellular senescence. It appears to function as a tumor suppressor as it is downregulated in colon and breast tumors.
Background Reference 1:
Hosono K, Sasaki T, Minoshima S, et al. Identification and characterization of a novel gene family YPEL in a wide spectrum of eukaryotic species. Gene 2004; 340: 31-43.
Background Reference 2:
Kelley K, Miller KR, Todd A, et al. YPEL3, a p53-regulated gene that induces cellular senescence. Cancer Res. 2010; 70:3566-75.
Background Reference 3:
Tuttle R, Simon R, Hitch DC, et al. Senescence-associated gene YPEL3 is downregulated in human tumors. Ann. Surg. Oncol. 2011; 18:1791-6.
Background Reference 4:
Tuttle R, Miller KR, Maiorano JN, et al. Novel senescence associated gene, YPEL3, is repressed by estrogen in ER+ mammary tumor cells and required for tamoxifen-induced cellular senescence. Int. J. Cancer 2011; epub.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6775

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369