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Western blot analysis of YPEL5 in A-20 cell lysate with YPEL5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of YPEL5 in A20 cells with YPEL5 antibody at 2.5 μg/mL.
Immunofluorescence of YPEL5 in A20 cells with YPEL5 antibody at 5 μg/mL.
Western blot analysis of YPEL5 in A-20 cell lysate with YPEL5 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

YPEL5 Antibody

CATALOG NUMBER: 6779

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
YPEL5 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human YPEL5.
The immunogen is located within the first 50 amino acids of YPEL5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
YPEL5 antibody can be used for detection of YPEL5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 17-208 - A-20 Cell Slide
Purification:
YPEL5 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
YPEL5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
YPEL5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
YPEL5
Additional Names:
YPEL5 Antibody: CGI-127, Protein yippee-like 5
Protein Accession Number:
NP_057145
Protein Gi Number:
7706341
Ncbi Gene Id Number:
51646
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
YPEL5 Antibody: YPEL5 (yippee-like 5) belongs to a family of five yippee-like proteins, all of which localize to the centrosome or mitotic spindle and are widely expressed in both adult and fetal tissue. This localization plus the fact that the family of human YPEL proteins share a high degree of sequence homology across species suggests that these proteins may have a conserved function involved in cell division. YPEL5 is expressed at the nucleus and centrosome during interphase; during mitosis, it localizes to the spindle poles, mitotic spindle, and spindle midzone during mitosis. Finally, during cytokinesis, YPEL5 is localized to the midbody. It is associated with the Ran Binding Protein in the Microtubule organizing center (RanBPM) and a related protein RanBP10.
Background Reference 1:
Hosono K, Sasaki T, Minoshima S, et al. Identification and characterization of a novel gene family YPEL in a wide spectrum of eukaryotic species. Gene 2004; 340: 31-43.
Background Reference 2:
Hosono K, Noda S, Shimuzu A, et al. YPEL5 protein of the YPEL gene family is involved in the cell cycle progression by interacting with two distinct proteins RanBPM and RanBP10. Genomics 2010; 96:102-11.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6779

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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