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Western blot analysis of CLEC7A in rat spleen tissue lysate with CLEC7A antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of CLEC7A in human spleen tissue with CLEC7A antibody at 5 μg/mL.
Immunofluorescence of CLEC7A in Jurkat cells with CLEC7A antibody at 20 μg/mL.
Flow Cytometry Validation of CLEC7A in Jurkat Cells 1X10^6/mL Jurkat cells were intracellularly stained with 2 μg/mL of CLEC7A antibody (red) or Rabbit IgG isotype control (black) at RT for 30 min, followed by PSFlo 488 conjugated goat anti-rabbit IgG secondary antibody staining (1:1000) at RT for 30 min. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100 in PBS.
Western blot analysis of CLEC7A in rat spleen tissue lysate with CLEC7A antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

CLEC7A Antibody

CATALOG NUMBER: 6833

Clonality:
Polyclonal
Tested Applications:
ELISA, FC, IHC-P, WB, ICC/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (88%)
Immunogen:
CLEC7A antibody was raised against a 17 amino acid synthetic peptide near the center of human CLEC7A.
The immunogen is located within amino acids 70 - 120 of CLEC7A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, FC, IHC-P, WB, ICC/IF
Application Note:
WB: 1-2 μg/mL; IHC-P: 5 μg/mL; ; ICC/IF: 20 μg/mL; FC: 2 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry, Immunofluorescence and Flow Cytometry in human samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of CLEC7A are known to exist.
Positive Control 1:
Cat. No. 1466 - Rat Spleen Tissue Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 27 kDa
Observed: 27 kDa
Purification:
CLEC7A Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CLEC7A Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CLEC7A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CLEC7A
Additional Names:
CLEC7A Antibody: BGR, CANDF4, DECTIN1, CLECSF12, BGR, UNQ539/PRO1082, C-type lectin domain family 7 member A, Beta-glucan receptor, DC-associated C-type lectin 1
Protein Accession Number:
NP_922938
Protein Gi Number:
37675373
Ncbi Gene Id Number:
64581
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CLEC7A Antibody: CLEC7A, also known as dectin-1, is a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily and is predominantly expressed on myeloid cells. It is a small glycoprotein type II membrane receptor with an extracellular C-type lectin-like domain fold and a cytoplasmic domain with an immunoreceptor tyrosine-based activation motif (ITAM). CLEC7A functions as a pattern-recognition receptor that recognizes a variety of beta-1,3-linked and beta-1,6-linked glucans from fungi and plants, and in this way plays a role in innate immune response. Upon fungal exposure, CLEC7A activates Syk tyrosine kinase, triggering a massive oxidative burst through the formation of reactive oxygen species.
Background Reference 1:
Taylor PR, Brown GD, Reid DM, et al. The beta-glucan receptor, dectin-1, is predominantly expressed on the surface of cells of the monocyte/macrophage and neutrophil lineages. J. Immunol. 2002; 169:3876-82.
Background Reference 2:
Ebner S, Sharon S, and Ben-Tal N. Evolutionary analysis reveals collective properties and specificity in the C-type lectin and lectin-like domain superfamily. Proteins 2003; 53:44-55.
Background Reference 3:
Kerrigan AM and Brown GD. Syk-coupled C-type lectin receptors that mediate cellular activation via single tyrosine based activation motifs. Immunol. Rev. 2010; 234:335-52.
Background Reference 4:
Underhill DM, Rossnagle E, Lowell CA, et al. Dectin-1 activates Syk tyrosine kinase in a dynamic subset of macrophages for reactive oxygen production. Blood 2005; 106:2543-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6833

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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