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Western blot analysis of LEMD2 in 293 cell lysate with LEMD2 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of LEMD2 in 3T3 cells with LEMD2 antibody at 2.5 μg/mL.
Immunofluorescence of LEMD2 in 3T3 cells with LEMD2 antibody at 20 μg/mL.
Western blot analysis of LEMD2 in 293 cell lysate with LEMD2 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

LEMD2 Antibody

CATALOG NUMBER: 6891

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
LEMD2 antibody was raised against a 16 amino acid synthetic peptide near the center of human LEMD2.
The immunogen is located within amino acids 220 - 270 of LEMD2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
LEMD2 antibody can be used for detection of LEMD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of LEMD2 are known to exist; this antibody will detect only the longer isoform.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Purification:
LEMD2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LEMD2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LEMD2 antibody can be stored at 4°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LEMD2
Additional Names:
LEMD2 Antibody: NET25, dJ482C21.1, LEM domain-containing protein 2, hLEM2
Protein Accession Number:
NP_851853
Protein Gi Number:
31044432
Ncbi Gene Id Number:
221496
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LEMD2 Antibody: The LEM (lamina-associated polypeptide-emerin-MAN1) domain is a motif shared by a group of lamin-interacting proteins of the inner nuclear membrane (INM) and nucleoplasm. One such protein, LEMD2 (LEM domain-containing protein 2), is a lamina-associated protein in the INM involved in nuclear structure organization, containing an N-terminal LEM motif, two transmembrane domains, and a MAN1-Src1p C-terminal (MSC) domain. LEMD2 is a truncated paralog of MAN1, lacking the MAN1-specific C-terminal RNA-recognition motif, but like MAN1 is required for myogenic differentiation. Mutations in LEMD2 are thought be among the possible causes of muscle disease.
Background Reference 1:
Brachner A, Reipert S, Foisner R, et al. LEM2 is a novel MAN1-related inner nuclear membrane protein associated with A-type lamins. J. Cell Sci. 2005; 118:5797-810
Background Reference 2:
Huber MD, Guan T, and Gerace L. Overlapping functions of nuclear envelope proteins NET25 (Lem2) and Emerin in regulation of extracellular signal-regulated kinase signaling in myoblast differentiation. Mol. Cell. Biol. 2009; 29:5718-28.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6891

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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