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Western blot analysis of BMP15 in human testis tissue lysate with BMP15 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of BMP15 in 3T3 cells with BMP15 antibody at 2.5 μg/mL.
Immunofluorescence of BMP15 in 3T3 cells with BMP15 antibody at 20 μg/mL.
Western blot analysis of BMP15 in human testis tissue lysate with BMP15 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

BMP15 Antibody

CATALOG NUMBER: 6909

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (75%), Sheep: (75%)
Immunogen:
BMP15 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human BMP15.
The immunogen is located within amino acids 30 - 80 of BMP15.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
BMP15 antibody can be used for detection of BMP15 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
It is closely related to GDF9.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Predicted Molecular Weight:
Predicted: 45 kDa
Observed: 50 kDa
Purification:
BMP15 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BMP15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BMP15 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
BMP15
Additional Names:
BMP15 Antibody: ODG2, POF4, GDF9B, Bone morphogenetic protein 15, BMP-15
Protein Accession Number:
NP_005439
Protein Gi Number:
257743454
Ncbi Gene Id Number:
9210
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
BMP15 Antibody: BMP15, or GDF9B is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily that are involved in embryonic development and adult tissue homeostasis. BMP15 is expressed exclusively in the oocyte. It is an oocyte-specific growth/differentiation factor that may be involved in oocyte maturation and follicular development. Defects in BMP15 are the cause of ovarian dysgenesis type 2. BMP15 could be used as an oogenesis marker to track human amniotic fluid stem cells differentiation into the oocyte-like cells.
Background Reference 1:
Massagué J. The transforming growth factor-beta family. Ann. Rev. Cell. Biol. 1990; 6:597-641
Background Reference 2:
Aaltonen J, Laitinen MP, Vuojolainen K, et al. Human growth differentiation factor 9 (GDF-9) and its novel homolog GDF-9B are expressed in oocytes during early foliculogenesis. J. Clin. Endocrinol. Metab. 1999; 84:2744-50.
Background Reference 3:
Laitinen M, Vuojolainen K, Jaatinen R, et al. A novel growth differentiation factor-9 (GDF-9) related factor is coexpressed with GDF-9 in mouse oocytes during folliculogenesis. Mech. Dev. 1998; 78:135-40.
Background Reference 4:
Galloway SM, Gregan SM, Wilson T et al. Bmp15 mutations and ovarian function. Mol. Cell Endocrinol. 2002; 191:15-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6909

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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