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Western blot analysis of RAB21 in mouse kidney tissue lysate with RAB21 antibody at 1 μg/mL.
Immunofluorescence of RAB21 in human kidney tissue with RAB21 antibody at 20 μg/mL.
Western blot analysis of RAB21 in mouse kidney tissue lysate with RAB21 antibody at 1 μg/mL.
Trial Size

RAB21 Antibody

CATALOG NUMBER: 6927

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (95%)
Immunogen:
RAB21 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human RAB21.
The immunogen is located within the last 50 amino acids of RAB21.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
RAB21 antibody can be used for detection of RAB21 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
This antibody is predicted to not cross-react with other Ras-related proteins
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
RAB21 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RAB21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RAB21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RAB21
Additional Names:
RAB21 Antibody: KIAA0118, Ras-related protein Rab-21
Protein Accession Number:
NP_055814
Protein Gi Number:
7661922
Ncbi Gene Id Number:
23011
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RAB21 Antibody: RAB21 is a member of a subfamily of small GTP-binding protein genes of the Ras superfamily that plays an important role in intracellular vesicular targeting. In non-polarized Caco-2 cells, RAB21 showed a general endoplasmic reticulum (ER)-like localization, while in polarized cells, RAB21 localized to apical vesicles. RAB21 has been shown to associate with integrin subunits and to be important for receptor entry into cells via RAB5/RAB21 endosomes. The GTPase-activating protein p120RasGAP regulates cell motility by controlling the return of the endocytosed integrins to the plasma membrane by competing with RAB21 for binding to overlapping sites on the α-tail of endocytosed integrin.
Background Reference 1:
Opdam FJ, Kamps G, Croes H, et al. Expression of Rab small GTPases in epithelial Caco-2 cells: Rab21 is an apically located GTP-binding protein in polarised intestinal epithelial cells. Eur. J. Cell Biol. 2000; 79:308-16.
Background Reference 2:
Pellinen T and Ivaska J. Integrin traffic. J. Cell Sci. 2006; 119:3723-31.
Background Reference 4:
Mai A, Veltel S, Pellinen T, et al. Competitive binding of Rab21 and p120RasGAP to integrins regulates receptor traffic and migration. J. Cell Biol. 2011; 194:291-306.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6927

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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