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Western blot analysis of ZF21 in 3T3 cell tissue lysate with ZF21 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of ZF21 in human kidney tissue with ZF21 antibody at 20 μg/mL.
Western blot analysis of ZF21 in 3T3 cell tissue lysate with ZF21 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ZF21 Antibody

CATALOG NUMBER: 6935

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (93%)
Immunogen:
ZF21 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ZF21.
The immunogen is located within the first 50 amino acids of ZF21.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
ZF21 antibody can be used for detection of ZF21 by Western blot at 1 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of ZF21 are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Purification:
ZF21 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ZF21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ZF21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ZFYVE21
Additional Names:
ZF21 Antibody: ZF21, Zinc finger FYVE domain-containing protein 21, ZF21
Protein Accession Number:
NP_001185882
Protein Gi Number:
312147341
Ncbi Gene Id Number:
79038
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ZF21 Antibody: ZF21 was initially identified as protein that could bind to the cytoplasmic tail of MT1-MMP (Membrane-type 1 matrix metalloproteinase), a potent invasion-promoting protease. ZF21 is a member of a protein family characterized by the presence of a phosphatidylinositol 3-phosphate-binding FYVE domain and regulates focal adhesions (FAs) and cell movement. Knockdown of ZF21 expression resulted in a delay of FA disassembly following induction of synchronous disassembly of FAs by nocodazole treatment, suggesting that ZF21 is involved in FA disassembly. ZF21 contains a noncanonical pleckstrin homology domain that is a possible therapeutic target to treat metastatic cancer.
Background Reference 1:
Uekita T, Gotoh I, Kinoshita T, et al. Membrane-type 1 matrix metalloproteinase cytoplasmic tail-binding protein-1 is a new member of the Cupin superfamily. A possible multifunctional protein acting as an invasion suppressor down-regulated in tumors. J. Biol. Chem. 2004; 279:12734-43.
Background Reference 2:
Nagano M, Hoshino D, Sakamoto T, et al. ZF21 protein regulates cell adhesion and motility. J. Biol. Chem. 2010; 285:21013-22.
Background Reference 3:
Nagano M, Hoshino D, Koshiba S, et al. ZF21 protein, a regulator of the disassembly of focal adhesions and cancer metastasis, contains a novel noncanonical pleckstrin homology domain. J. Biol. Chem. 2011; 286:31598-609.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6935

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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