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Western blot analysis of TCIRG1 in EL4 cell lysate with TCIRG1 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of TCIRG1 in EL4 cell lysate with TCIRG1 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

TCIRG1 Antibody

CATALOG NUMBER: 6973

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TCIRG1 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCIRG1.
The immunogen is located within amino acids 70 - 120 of TCIRG1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
TCIRG1 antibody can be used for detection of TCIRG1 by Western blot at 0.5 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificity:
At least two alternatively spliced isoforms are known to exist; this antibody will only detect the larger isoform.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Predicted Molecular Weight:
91 kDa
Purification:
TCIRG1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TCIRG1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TCIRG1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TCIRG1
Additional Names:
TCIRG1 Antibody: a3, Stv1, Vph1, Atp6i, OC116, OPTB1, TIRC7, ATP6N1C, ATP6V0A3, OC-116kDa, V-type proton ATPase 116 kDa subunit a isoform 3, Osteoclastic proton pump 116 kDa subunit, V-ATPase 116 kDa isoform a3
Protein Accession Number:
NP_006010
Protein Gi Number:
19924145
Ncbi Gene Id Number:
10312
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TCIRG1 Antibody: The T-cell immune regulator 1 (TCIRG1) is the a3 subunit of the vacuolar-type proton transporting ATPase (V-ATPase), a proton pump in late endosomes and lysosomes that functions in the luminal acidification of these organelles. Genetic defects in the gene encoding this protein a responsible for a severe form of autosomal recessive osteopetrosis, a condition that leads to increased bone density, decreased bone strength, and inflammation in bone tissues. TCIRG1 is also required for the normal secretion of insulin and the bacteria-killing function of macrophages.
Background Reference 1:
Toyomura T, Oka T, Yamaguchi C, et al. Three subunit a isoforms of mouse vacuolar H+-ATPase. Preferential expression of the a3 isoform during osteoclast differentiation. J. Biol. Chem. 2000; 275:8760-5
Background Reference 2:
Kornak U, Schulz A, Friedrich W, et al. Mutations in the a3 subunit of the vacuolar H+-ATPase cause malignant osteopetrosis. Hum. Mol. Genet. 2000; 9:2059-63.
Background Reference 3:
Sun-Wada GH, Toyomura T, Kawamura N, et al. The a3 isoform of V-ATPase regulates insulin secretion from pancreatic b-cells. J. Cell Sci. 2006; 119:4531-40.
Background Reference 4:
Sun-Wada GH, Tabata H, Kawamura N, et al. Direct recruitment of H+-ATPase from lysosomes for phagocytic acidification. J. Cell Sci. 2009; 122:2504-13.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6973

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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