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Western blot analysis of RGS22 in Jurkat cell lysate with RGS22 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of RGS22 in human testis tissue with RGS22 antibody at 20 μg/mL.
Western blot analysis of RGS22 in Jurkat cell lysate with RGS22 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

RGS22 Antibody

CATALOG NUMBER: 7001

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
RGS22 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human RGS22.
The immunogen is located within amino acids 870 - 920 of RGS22.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
RGS22 antibody can be used for detection of RGS22 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of RGS22 are known to exist; this antibody will detect the three longest isoforms. RGS22 antibody is predicted to not cross-react with other RGS proteins
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Predicted Molecular Weight:
139 kDa
Purification:
RGS22 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
RGS22 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
RGS22 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
RGS22
Additional Names:
RGS22 Antibody: CT145, PRTD-NY2, Regulator of G-protein signaling 22, RGS22
Protein Accession Number:
NP_056483
Protein Gi Number:
158534077
Ncbi Gene Id Number:
26166
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
RGS22 Antibody: Regulator of G-protein signaling (RGS) proteins contain an 120 amino acid conserved domain, termed the RGS domain, that acts as a GTPase-activating protein that acts to reduce the signal transmitted by the receptor-activated G-alpha subunit. RGS22 is a recently identified member of this family that localizes to the testis and can interact with guanine nucleotide binding proteins alpha 11, 12, and 13 (GNA11, GNA12, and GNA13). While RGS22 has been postulated to play a role in spermiogenesis in the testis, it is also expressed in several cancer cell lines with an epithelial origin and associated with cancer metastasis. Its overexpression in a highly metastatic cancer causes a decrease in cell migration and a reduction of the invasive potential of the cells, suggesting that RGS22 may be a potential prognostic biomarker for metastasis.
Background Reference 1:
De Vries L, Mousli M, Wurmser A, et al. GAIP, a protein that specifically interacts with the G protein G alpha i3, is a member of a protein family with a highly conserved core domain. Proc. Natl. Acad. Sci. USA 1995; 92:11916-20.
Background Reference 2:
Berman DM, Wilkie TM, and Gilman AG. GAIP and RGS4 are GTP-ase activating proteins for the Gi subfamily of G protein alpha subunits. Cell 1996; 86:445-52.
Background Reference 3:
Hu Y, Xing J, Chen L, et al. RGS22, a novel testis-specific regulator of G-protein signaling involved in human and mouse spermiogenesis along with GNA12/13 subunits. Biol. Reprod. 2008; 79:1021-9
Background Reference 4:
Hu Y, Xing J, Wang L, et al. RGS22, a novel cancer/testis antigen, inhibits epithelial cell invasion and metastasis. Clin. Exp. Metastasis 2011; 28:541-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7001

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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