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Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of DLK1 in HepG2 cells with DLK1 antibody at 2.5 μg/ml.
Immunofluorescence of DLK1 in HepG2 cells with DLK1 antibody at 20 μg/ml.
Western blot analysis of HepG2 in 293 cell lysate with DLK1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

DLK1 Antibody

CATALOG NUMBER: 7017

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
DLK1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human DLK1.
The immunogen is located within amino acids 260 - 310 of DLK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
DLK1 antibody can be used for detection of DLK1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of DLK1 are known to exist.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 17-011 - Hep G2 Cell Slide
Predicted Molecular Weight:
42 kDa
Purification:
DLK1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DLK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DLK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DLK1
Additional Names:
DLK1 Antibody: DLK, Protein delta homolog 1, pG2, DLK-1
Protein Accession Number:
NP_003827
Protein Gi Number:
74136023
Ncbi Gene Id Number:
8788
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DLK1 Antibody: The Delta-like 1 homolog (DLK1) is a transmembrane protein containing six epidermal growth factor repeats. It is involved in the differentiation of several cell types, including adipocytes and is also thought to be a tumor suppressor. The DLK1 gene is one of several imprinted genes located in a region on chromosome 14q32; certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). DLK1 is expressed from the paternal allele; a polymorphism within this gene has been associated with child and adolescent obesity.
Background Reference 1:
Smas CM and Sul HS. Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation. Cell 1993; 73:725-34.
Background Reference 2:
Sul HS. Mini-review: Pref-1: role in adipogenesis and mesenchymal cell fate. Mol. Endocrinol. 2009; 23:1717-25.
Background Reference 3:
Kawakami T, Chano T, Minami K, et al. Imprinted DLK1 is a putative tumor suppressor gene and inactivated by epimutation at the region upstream of GTL2 in human renal cell carcinoma. Hum. Mol. Genet. 2006; 15:821-30.
Background Reference 4:
Wermter AK, Scherag A, Meyre D, et al. Preferential reciprocal transfer of paternal/maternal DLK1 alleles to obese children: first evidence of polar overdomainance in human. Eur. J. Hum. Genet. 2008; 16:1126-34.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7017

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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