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Western blot analysis of PHLPP1 in SW480 cell lysate with PHLPP1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of PHLPP1 in SW480 cells with PHLPP1 antibody at 2.5 μg/ml.
Immunofluorescence of PHLPP1 in SW480 cells with PHLPP1 antibody at 5 μg/ml.
Western blot analysis of PHLPP1 in SW480 cell lysate with PHLPP1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

PHLPP1 Antibody

CATALOG NUMBER: 7019

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1.
The immunogen is located within amino acids 250 - 300 of PHLPP1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
PHLPP1 antibody can be used for detection of PHLPP1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least four isoforms are known to exist; this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.
Positive Control 1:
Cat. No. 1221 - SW480 Cell Lysate
Predicted Molecular Weight:
189 kDa
Purification:
PHLPP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PHLPP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PHLPP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PHLPP1
Additional Names:
PHLPP1 Antibody: SCOP, PHLPP, PLEKHE1, KIAA0606, SCOP, PH domain leucine-rich repeat-containing protein phosphatase 1, Pleckstrin homology domain-containing family E member 1, PH domain-containing family E member 1
Protein Accession Number:
NP_919431
Protein Gi Number:
291219891
Ncbi Gene Id Number:
23239
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PHLPP1 Antibody: PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.
Background Reference 1:
Brognard J and Newton AC. PHLiPPing the switch on Akt and protein kinase C signaling. Trends Endocrinol. Metab. 2008; 19:223-30.
Background Reference 2:
Gao T, Furnari F and Newton AC. PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth. Mol. Cell 2005; 18:13-24.
Background Reference 3:
Brognard J, Sierecki E, Gao T, et al. PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms. Mol. Cell 2007; 25:917-31
Background Reference 4:
Andreozzi F, Procopio C, Greco A, et al. Increased levels of the Akt-specific phosphatase PH domain leucine-rich repeat protein phosphatase (PHLPP)-1 in obese participants are associated with insulin resistance. Diabetologia 2011; 54:1879-87.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7019

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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