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Western blot analysis of CENPW in HeLa cell lysate lysate with CENPW antibody at (A) 0.5 and (B) 1 μg/mL.
Western blot analysis of CENPW in HeLa cell lysate lysate with CENPW antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

CENPW Antibody

CATALOG NUMBER: 6943

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
CENPW antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CENPW.
The immunogen is located within the first 50 amino acids of CENPW.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
CENPW antibody can be used for detection of CENPW by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of CENPW are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
kDa
Purification:
CENPW Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CENPW Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CENPW antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
CENPW
Additional Names:
CENPW Antibody: CUG2, CENP-W, C6orf173, CUG2, Centromere protein W, Cancer-up-regulated gene 2 protein
Protein Accession Number:
NP_001012525
Protein Gi Number:
60302883
Ncbi Gene Id Number:
387103
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
CENPW Antibody: CENPW was initially identified as a gene that was upregulated in multiple cancers, and whose overexpression in mouse fibroblast cells gave rise to distinct cancer-specific phenotypes. It was later found to be a nuclear protein that associated with CENPT, a component of CENPA nucleosome complex in the centromere, and is required for proper kinetochore function. CENPW also specifically interacts with the nucleolar phosphoprotein nucleophosmin, also known as B23 It has been suggested that nucleophosmin functions in the assembly of the kinetochore by interacting with CENPW during interphase. Overexpression of CENPW in the SKOV-3 human ovarian cancer cell line as well as in the zebrafish embryo led to apoptosis, suggesting that high levels of CENPW induces apoptotic cell death.
Background Reference 1:
Lee S, Gang J, Jeon SB, et al. molecular cloning and functional analysis of a novel oncogene, cancer-upregulated gene 2 (CUG2). Biochem. Biophys. Res. Commun. 2007; 360:633-9.
Background Reference 2:
Kim H, Lee M, Lee S, et al. Cancer-upregulated gene 2 (CUG2), a new component of centromere complex, is required for kinetochore function. Mol. Cells 2009; 27:697-701.
Background Reference 3:
Chun Y, Park B, Koh W, et al. New centromeric component CENT-W is an RNA-associated nuclear matrix protein that interacts with nucleophosmin/B23 protein. J. Biol. Chem. 2011; 286:42758-69.
Background Reference 4:
Lee S, Koh W, Kim HT, et al. Cancer-upregulated gene 2 (CUG2) overexpression induces apoptosis in SKOV-3 cells. Cell Biochem. Funct. 2010; 28:461-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6943

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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