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Western blot analysis of DDX60 in A20 cell lysate with DDX60 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of DDX60 in A20 cells with DDX60 antibody at 20 μg/mL.
Western blot analysis of DDX60 in A20 cell lysate with DDX60 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

DDX60 Antibody

CATALOG NUMBER: 6945

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
DDX60 antibody was raised against a 17 amino acid synthetic peptide near the center of human DDX60.
The immunogen is located within amino acids 1140 - 1190 of DDX60.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
DDX60 antibody can be used for detection of DDX60 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of DDX60 are known to exist; this antibody will detect all three isoforms.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 17-208 - A-20 Cell Slide
Predicted Molecular Weight:
Predicted: 125, 185, 188 kDa
Observed: 190 kDa
Purification:
DDX60 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DDX60 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DDX60 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
DDX60
Additional Names:
DDX60 Antibody: Probable ATP-dependent RNA helicase DDX60, DEAD box protein 60
Protein Accession Number:
NP_060101
Protein Gi Number:
222831595
Ncbi Gene Id Number:
55601
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DDX60 Antibody: DDX60 is a DEXD/H Box RNA helicase and the human homolog of the S. cerevisiae Ski2, a cofactor of RNA exosome. Expression of DDX60 increases after viral infection and binds to endogenous RIG-I, a cytoplasmic viral RNA sensor, as well as other RIG-I-like receptors such as MDA5 and LGP2. These proteins activate TBK1 and IKK-e, signaling IRF3 and NF-kB-responsive genes in the innate immune response pathway. DDX60 is required for the RIG-I- or MDA5-dependent response to viral infection, but is dispensable for TLR3-mediated signaling.
Background Reference 1:
Miyashita M, Oshiumi H, Matsumoto M, et al. DDX60, a DEXD/H box helicase, is a novel antiviral factor promoting RIG-I-like receptor-mediated signaling. Mol. Cell. Biol. 2011; 31:3802-19.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6945

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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