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Western blot analysis of LMX1B in A-20 cell lysate with LMX1B antibody at (A) 1 and (B) 2 μg/mL.
Western blot analysis of LMX1B in A-20 cell lysate with LMX1B antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

LMX1B Antibody

CATALOG NUMBER: 7089

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rabbit polyclonal LMX1B antibody was raised against a 17 amino acid peptide near the carboxy terminus of human LMX1B.
The immunogen is located within amino acids 280 - 330 of LMX1B.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
LMX1B antibody can be used for detection of LMX1B by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificity:
At least three isoforms are known to exist. This antibody is predicted to not cross-react with LMX1A.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Positive Control 2:
Cat. No. 10-301 - Human Brain Tissue Slide
Predicted Molecular Weight:
45 kDa
Purification:
LMX1B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
LMX1B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
LMX1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
LMX1B
Additional Names:
LMX1B Antibody: NPS1, LMX1.2, LIM homeobox transcription factor 1-beta
Protein Accession Number:
NP_001167617
Protein Gi Number:
292494913
Ncbi Gene Id Number:
4010
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
LMX1B Antibody: The LIM homeobox transcription factor 1B (LMX1B) belongs to the LIM-homeodomain family. Members of this family are known to be important for pattern formation during development. LMX1B regulates mid-hindbrain patterning; LMX1B-null mice embryos have a severe reduction in the number of midbrain dopaminergic neurons compared to wild-type. While LMX1B appears to be important for both the development and the survival of dopamine neurons, the related LMX1A is crucial for the differentiation of these cells. However, LMX1A and LMX1B function cooperatively to regulated the proliferation, specification and differentiation of midbrain dopaminergic neuronal progenitors.
Background Reference 1:
Hobert O and Westphal H. Functions of LIM-homeobox genes. Trends Genet. 2000; 16:75-83.
Background Reference 2:
Nakatani T, Kumai M, Mizuhara E, et al. Lmx1a and Lmx1b cooperate with Foxa2 to coordinate the specification of dopaminergic neurons and control of floor plate cell differentiation in the developing mesencephalon. Dev. Biol. 2010; 339:101-13.
Background Reference 3:
Guo C, Qiu HY, Huang Y, et al. Lmx1b is essential for FGF8 and Wnt1 expression in the isthmic organizer during tectum and cerebellum development in mice. Development 2007; 134:317-25.
Background Reference 4:
Chizhikov VV and Millen KJ. Control of roof plate formation by LMX1A in the developing spinal cord. Development 2004; 131:2693-705.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7089

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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