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Western blot analysis of GDF1 in rat lung tissue lysate with GDF1 antibody at (A) 1 and (B) 2 μg/mL.
Immunofluorescence of GDF1 in human lung tissue with GDF1 antibody at 20 μg/mL.
Western blot analysis of GDF1 in rat lung tissue lysate with GDF1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

GDF1 Antibody

CATALOG NUMBER: 7093

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rabbit polyclonal GDF1 antibody was raised against a 16 amino acid peptide near the amino terminus of human GDF1.
The immunogen is located within amino acids 30 - 80 of GDF1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
GDF1 antibody can be used for detection of GDF1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
GDF1 antibody is predicted to not cross-react with any other members of the growth differentiation factor family.
Positive Control 1:
Cat. No. 1462 - Rat Lung Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
41 kDa
Purification:
GDF1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GDF1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GDF1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
GDF1
Additional Names:
GDF1 Antibody: RAI, DORV, DTGA3GDF-1
Protein Accession Number:
NP_001483
Protein Gi Number:
110349792
Ncbi Gene Id Number:
2657
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
GDF1 Antibody: Growth differentiation factors (GDFs) are members of the transforming growth factor (TGF) superfamily that is involved in embryonic development and adult tissue homeostasis. GDF1 was initially identified as a temporally expressed gene in the mouse central nervous system during embryonic development, with only one isoform detected in adult tissues. GDF1 is required for left-right patterning during development and directly interacts with Nodal, another member of the TGF-beta superfamily. It has been suggested that GDF1 regulates the activity and signaling range of Nodal through direct interaction.
Background Reference 1:
Massague J. 1990. The transforming growth factor-beta family. Ann. Rev. Cell Biol. 6:597-641.
Background Reference 2:
Lee SJ. Expression of growth/differentiation factor 1 in the nervous system: conservation of a bicistronic structure. Proc. Natl. Acad. Sci. USA 1991; 88:4250-4.
Background Reference 3:
Rankin CT, Bunton T, Lawler AM, et al. Regulation of left-right patterning in mice by growth/differentiation factor-1. Nat. Genet. 2000; 24:262-5.
Background Reference 4:
Tanaka C, Sakuma R, Nakamura T, et al. Long-range action of Nodal requires interaction with GDF1. Genes Dev. 2007; 21:3272-82.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7093

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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