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Western blot analysis of FOXH1 in human liver tissue lysate with FOXH1 antibody at 1 μg/mL.
Immunofluorescence of FOXH1 in human liver tissue with FOXH1 antibody at 20 μg/mL.
Western blot analysis of FOXH1 in human liver tissue lysate with FOXH1 antibody at 1 μg/mL.
Trial Size

FOXH1 Antibody

CATALOG NUMBER: 7095

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rabbit polyclonal FOXH1 antibody was raised against a 19 amino acid peptide near the amino terminus of human FOXH1.
The immunogen is located within the first 50 amino acids of FOXH1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
FOXH1 antibody can be used for detection of FOXH1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
FOXH1 antibody is predicted to not cross-react with any other members of the forkhead box family.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Predicted Molecular Weight:
40 kDa
Purification:
FOXH1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FOXH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FOXH1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures
Ncbi Official Symbol:
FOXH1
Additional Names:
FOXH1 Antibody: FAST1, FAST-1, FAST1, FAST2, Forkhead box protein H1, Forkhead activin signal transducer 1, Fast-1
Protein Accession Number:
NP_003914
Protein Gi Number:
4503657
Ncbi Gene Id Number:
8928
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FOXH1 Antibody: FOXH1 is a member of the Forkhead-box (FOX) family of transcription factors and is the human homolog of Xenopus forkhead activin signal transducer-1. FOXH1 plays an important role in mediating TGF-beta/activin signals through its interaction with the SMAD2-SMAD4 complex. It is expressed primarily during early development and localizes in the cytoplasm until it forms a complex with the SMAD proteins at which time it translocates to the nucleus to positively or negatively affect gene expression.
Background Reference 1:
Chen X, Weisberg E, Fridmacher V, et al. Smad and FAST-1 in the assembly of activin-responsive factor. Nature 1997; 389:85-9.
Background Reference 2:
Zhou S, Zewel L, Lengauer C, et al. Characterization of human FAST-1, a TGF beta and activin signal transducer. Mol. Cell 1998; 2:121-7.
Background Reference 3:
Attisano L, Silvestri C, Izzi L, et al. The transcriptional role of Smads and FAST (FoxH1) in TGFbeta and activin signaling. Mol. Cell. Endocrinol. 2001; 180:3-11.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7095

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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