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Western blot analysis of WIPI2 in human testis tissue lysate with WIPI2 antibody at 1 μg/mL
Western blot analysis of WIPI2 in human testis tissue lysate with WIPI2 antibody at 1 μg/mL
Trial Size

WIPI2 Antibody

CATALOG NUMBER: 7103

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Rabbit polyclonal WIPI2 antibody was raised against a 17 amino acid peptide near the amino terminus of human WIPI2.
The immunogen is located within the first 50 amino acids of WIPI2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WIPI2 antibody can be used for detection of WIPI2 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
WIPI2 antibody is human and rat reactive. Multiple isoforms of WIPI2 are known to exist. WIPI2 antibody is predicted to not cross-react with WIPI1.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Predicted Molecular Weight:
50 kDa
Purification:
WIPI2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
WIPI2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
WIPI2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
WIPI2
Additional Names:
WIPI2 Antibody: Atg21, ATG18B, CGI-50, WIPI-2, WD repeat domain phosphoinositide-interacting protein 2, WIPI49-like protein 2
Protein Accession Number:
NP_056425
Protein Gi Number:
7661580
Ncbi Gene Id Number:
26100
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
WIPI2 Antibody: WD repeat proteins play a role in many essential biologic functions, regulating the assembly of multiprotein complexes by presenting a beta-propeller platform for simultaneous and reversible protein-protein interactions. WIPI2, also known as ATG18B or ATG21, is a human homolog to yeast ATG18 and contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. It is recruited to early autophagosomal structures along with Atg16L and ULK1 and is required for the formation of LC3-positive autophagosomes. Along with the highly related WIPI1, WIPI2 is found at the plasma membrane in addition to autophagosomal membranes.
Background Reference 1:
Smith TF. Diversity of WD-repeat proteins. Subcell. Biochem. 2008; 48:20-30.
Background Reference 2:
Polson HE, de Lartique J, Rigden DJ, et al. Mammalian ATG18 (WIPI2) localizes to moegasome-anchored phagophores and positively regulates LC3 lipidation. Autophagy 2010; 6:506-22.
Background Reference 3:
Proikas-Cezanne T and Robenek H. Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes. J. Cell. Mol. Med. 2011; 15:2007-10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7103

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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