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Western blot analysis of FOXP3 in A549 cell lysate with FOXP3 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of FOXP3 in human brain tissue with FOXP3 antibody at 5 μg/mL.
Immunofluorescence of FOXP3 in human brain tissue with FOXP3 antibody at 20 μg/mL.<br><br>Green: FOXP3 Antibody (7187) <br> Blue: DAPI staining
Western blot analysis of FOXP3 in A549 cell lysate with FOXP3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

FOXP3 Antibody

CATALOG NUMBER: 7187

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rabbit polyclonal FOXP3 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human FOXP3.
The immunogen is located within the last 50 amino acids of FOXP3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
FOXP3 antibody can be used for detection of FOXP3 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Predicted Molecular Weight:
Predicted: 47 kDa
Purification:
FOXP3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FOXP3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FOXP3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
FOXP3
Additional Names:
FOXP3 Antibody: JM2, AIID, IPEX, PIDX, XPID, DIETER, JM2, Forkhead box protein P3, Scurfin
Protein Accession Number:
NP_054728
Protein Gi Number:
31982943
Ncbi Gene Id Number:
50943
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FOXP3 Antibody: FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators. FOXP3 acts as a repressor of transcription and regulates T cell activation, with its overexpression in CD4 T cells leading to an attenuation of activation-induced cytokine production and proliferation. In regulatory T (Treg) cells, FOXP3 is essential for Treg suppressor function and its expression leads to the repression of IL-17 expression. Genetic mutations involving FOXP3 are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome.
Background Reference 1:
Disruption of a new forkhead/winged-helix protein, scurfin, results in the fatal lymphoproliferative disorder of the scurfy mouse. Nat. Genet. 2001; 27:68-73
Background Reference 2:
Schubert LA, Jeffery E, Zhang Y, et al. Scurfin (FOXP3) acts as a repressor of transcription and regulates T cell activation. J. Biol. Chem. 2001; 276:37672-9.
Background Reference 3:
Gavin MA, Rasmussen JP, Fontenot JD, et al. Foxp3-dependent programme of regulatory T-cell differentiation. Nature 2007; 445:771-5
Background Reference 4:
Kobayashi I, Shiari R, Yamada M, et al. Novel mutations of FOXP3 in two Japanese patients with immune dysregulation, polyendocrinopathy, enteropathy, X linked syndrome (IPEX). J. Med. Genet. 2001; 38:874-6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7187

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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