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Western blot analysis of TSC22D3 in human small intestine tissue lysate with TSC22D3 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TSC22D3 in human small intestine tissue with TSC22D3 antibody at 5 μg/ml.
Immunofluorescence of TSC22D3 in human small intestine tissue with TSC22D3 antibody at 20 μg/ml.
Western blot analysis of TSC22D3 in human small intestine tissue lysate with TSC22D3 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TSC22D3 Antibody

CATALOG NUMBER: 7221

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Rabbit polyclonal TSC22D3 antibody was raised against a 16 amino acid peptide near the amino terminus of human TSC22D3.
The immunogen is located within amino acids 40 - 90 of TSC22D3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TSC22D3 antibody can be used for detection of TSC22D3 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1308 - Human Small Intestine Tissue Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 22 kDa
Observed: 23 kDa
Purification:
TSC22D3 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TSC22D3 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TSC22D3 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures”
Ncbi Official Symbol:
TSC22D3
Additional Names:
TSC22D3 Antibody: DIP, GILZ, hDIP, DSIPI, TSC-22R, TSC22 domain family protein 3, DSIP-immunoreactive peptide, Protein DIP
Protein Accession Number:
NP_932174
Protein Gi Number:
37622903
Ncbi Gene Id Number:
1831
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TSC22D3 Antibody: The TSC22 domain family member 3 protein (TSC22D3) is a leucine zipper protein that functions as a transcriptional regulator. The expression of TSC22D3 is stimulated by glucocorticoids and IL-10 and is thought to play a key role in the anti-inflammatory and immunosuppressive effects of these molecules. TSC22D3 can physically interact with and inhibit the activities of key inflammatory signaling mediators such NF-κB and AP-1. TSC22D3 functions as a transcriptional co-activator for various nuclear receptors and NF-κB. It has also been shown to be involved in the differentiation of mesenchymal stem cells towards osteoblasts and bone formation.
Background Reference 1:
Riccardi C, Cifone MG, and Migliorati G. Glucocorticoid hormone-induced modulation of gene expression and regulation of T-cell death: role of GITR and GILZ, two dexamethasone-induced genes. Cell Death Differ. 1999; 6:1182-9.
Background Reference 2:
Berrebi D, Bruscoli S, Cohen N, et al. Synthesis of glucocorticoid-induced leucine zipper (GILZ) by macrophages: an anti-inflammatory and immunosuppressive mechanism shared by glucocorticoids and IL-10. Blood 2003; 101:729-38
Background Reference 3:
Ayroldi E, Migliorati G, Bruscoli S, et al. Modulation of T-cell activation by the glucocorticoid-induced leucine zipper factor via inhibition of nuclear factor kappaB. Blood 2001; 98:743-53.
Background Reference 4:
Mittelstadt PR and Ashwell JD. Inhibition of AP-1 by the glucocorticoid-inducible protein GILZ. J. Biol. Chem. 2001; 276:29603-10.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7221

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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