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Western blot analysis of NELF in Hela cell lysate with NELF antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of NELF in rat brain tissue with NELF antibody at 2.5 μg/ml.
Immunofluorescence of NELF in rat brain tissue with NELF antibody at 20 μg/ml.
Immunohistochemistry of NELF in mouse brain tissue with NELF Antibody at 5 μg/mL.
Immunofluorescence of NELF in mouse brain tissue with NELF Antibody at 20 μg/mL.<br><br>Green: NELF antibody (7225) <br> Red: Phylloidin staining<br> Blue: DAPI staining
Western blot analysis of NELF in Hela cell lysate with NELF antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

NELF Antibody

CATALOG NUMBER: 7225

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Rabbit polyclonal NELF antibody was raised against a 17 amino acid peptide near the center of human NELF.
The immunogen is located within amino acids 230 - 280 of NELF.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
NELF antibody can be used for detection of NELF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
Predicted: 58 kDa
Purification:
NELF Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NELF Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NELF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
NELF
Additional Names:
NELF Antibody: HH9, NELF, NMDA receptor synaptonuclear signaling and neuronal migration factor, Nasal embryonic luteinizing hormone-releasing hormone factor, Nasal embryonic LHRH factor
Protein Accession Number:
NP_056352
Protein Gi Number:
46397396
Ncbi Gene Id Number:
26012
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
NELF Antibody: NELF (nasal embryonic luteinizing hormone-releasing hormone factor) is a 530 amino acid transcription factor involved in the migration of LHRH neurons, outgrowth of olfactory axons and suppression of transcription elongation. NELF is found in the peripheral and central nervous system during embryonic development, and is highly expressed in adult testis, kidney and brain. Known to couple NMDA receptor signaling to the nucleus, NELF knockdown impaired GnRH neuronal migration of NLT cells in vitro and the gene encoding NELF has been linked to the development of Idiopathic hypogonadotropic hypogonadism (IHH), a disorder resulting in impaired pubertal maturation and reproductive function.
Background Reference 1:
Kramer PR and Wray S. Novel gene expressed in nasal region influences outgrowth of olfactory axons and migration of luteinizing hormone-releasing hormone (LHRH) neurons. Genes Dev. 2000; 14:1824-34.
Background Reference 2:
Yung TM, Narita T, Komori T, et al. Cellular dynamics of the negative transcription elongation factor NELF. Exp. Cell Res. 2009; 315:1693-705.
Background Reference 3:
Xu N, Bhagavath B, Kim HG, et al. NELF is a nuclear protein involved in hypothalamic GnRH neuronal migration. Mol. Cell Endocrinol. 2010; 319:47-55.
Background Reference 4:
Miura K, Acierno JS and Seminara SB. Characterization of the human nasal embryonic LHRH factor gene, NELF, and a mutation screening among 65 patients with idiopathic hypogonadotropic hypogonadism (IHH). J. Hum. Genet. 2004; 49: 265-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7225

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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