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Western blot analysis of FOXA2 in human bladder tissue lysate with FOXA2 antibody at 1 μg/mL.
Immunofluorescence of FOXA2 in human bladder tissue with FOXA2 antibody at 20 μg/ml.
Immunohistochemistry of FOXA2 in mouse brain tissue with FOXA2 Antibody at 5 μg/mL.
Immunofluorescence of FOXA2 in mouse brain tissue with FOXA2 Antibody at 20 μg/mL. <br><br>Green: FOXA2 antibody (7257) <br> Red: Phylloidin staining<br> Blue: DAPI staining
Western blot analysis of FOXA2 in human bladder tissue lysate with FOXA2 antibody at 1 μg/mL.
Trial Size

FOXA2 Antibody

CATALOG NUMBER: 7257

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (94%), Mouse: (94%)
Immunogen:
FOXA2 antibody was raised against a 16 amino acid peptide near the center of human FOXA2 .
The immunogen is located within amino acids 280 - 330 of FOXA2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
FOXA2 Antibody can be used for detection of FOXA2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of FOXA2 are known to exist; this antibody will detect both isoforms. FOXA2 antibody is predicted to not cross-react with other members of the FOXA protein family.
Positive Control 1:
Cat. No. 1310 - Human Bladder Tissue Lysate
Predicted Molecular Weight:
Predicted: 51 kDa
Observed: 54 kDa
Purification:
FOXA2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
FOXA2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
FOXA2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
FOXA2
Additional Names:
FOXA2 Antibody: HNF3B, TCF3B, HNF3B, Hepatocyte nuclear factor 3-beta, Forkhead box protein A2, HNF-3-beta
Protein Accession Number:
NP_068556
Protein Gi Number:
194394143
Ncbi Gene Id Number:
3170
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
FOXA2 Antibody: FOXA2 is one of three members of the FOXA family, a subset of the forkhead family of transcription factors which play vital roles in development. FOXA2 was initially identified through library screening as a closely related homolog of FOXA1. Both FOXA2 and FOXA1 act as transcriptional activators in adult liver and also play a role in body axis formation, neural tube patterning and definitive endoderm formation during gastrulation.
Background Reference 1:
Hannenhalli S and Kaestner KH. The evolution of Fox genes and their role in development and disease. Nat. Rev. Genet. 2009; 10:233-40.
Background Reference 2:
Lai E, Prezioso VR, Tao WF, et al. Hepatocyte nuclear factor 3 alpha belongs to a gene family in mammals that is homologous to the Drosophila homeotic gene fork head. Genes Dev. 1991; 5:416-27.
Background Reference 3:
Sasaki H and Hogn BL. Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo. Dev. 1993; 118:47-59.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7257

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$35 Dry Ice Shipment fee may be required on some items.

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