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<strong>Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: KREMEN2, 7263 (2 μg/mL), KREMEN2, 64-235 (1 μg/mL) and beta-actin, 3779 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 Western Blot Validation in Human Cell Lines</strong><br>Loading: 15 μg of lysates per lane.Antibodies: KREMEN2, 7263 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 Western Blot Validation in Mouse Spleen Tissue Lysate</strong><br>Loading: 15 μg of lysates per lane.Antibodies: KREMEN2 7263 (Lane 1: 1 μg/mL and Lane 2: 2 μg/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunocytochemistry Validation of KREMEN2 in HeLa Cells</strong><br>Immunocytochemical analysis of HeLa cells using anti-KREMEN2 antibody (7263) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: KREMEN2, 7263 (2 μg/mL), KREMEN2, 64-235 (1 μg/mL) and beta-actin, 3779 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
IAV
Trial Size

KREMEN2 Antibody

CATALOG NUMBER: 7263

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-KREMEN2 antibody (7263) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human KREMEN2.
The immunogen is located within amino acids 390-440 of KREMEN2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, WB
Application Note:
WB: 1-2 μg/mL; ICC: 5 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of KREMEN2 are known to exist. KREMEN2 antibody is predicted to not cross-react with other Kremen protein family members.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 49kD
Observed: 49kD
Validation:

Independent Antibody Validation in Cell lines (Figure 1) shows similar KREMEN2 expression profile in human, mouse and rat cell lines detected by two independent anti-KREMEN2 antibodies that recognize different epitopes, 7263 against C-terminus domain and 64-235 against central domain. KREMEN2 proteins are detected in most of the tested cell lines at different expression levels by the two independent antibodies.

Isoforms:
Human KREMEN2 has 6 isoforms, including isoform 1 (462aa, 49kD), isoform 2 (424aa, 45kD), isoform 3 (420aa, 44kD), isoform 4 (399aa, 42kD), isoform 5 (423aa, 45kD), and isoform 6 (381aa, 40kD). Mouse KREMEN2 has one isoform (461aa, 49kD) and rat KREMEN2 also has one isoform (468aa, 50kD). 7263 can detect human, mouse and rat.
Purification:
KREMEN2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KREMEN2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KREMEN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
KREMEN2
Additional Names:
KREMEN2 Antibody: KRM2, KRM2, Kremen protein 2, Dickkopf receptor 2
Protein Accession Number:
NP_757384
Protein Gi Number:
27437008
Ncbi Gene Id Number:
79412
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KREMEN2 Antibody: Kremen (Kringle containing protein marking the eye and the nose) proteins are type I transmembrane proteins that contain extracellular kringle, WSC and CUB domains and an intracellular region without any conserved motifs. Kremens bind a subset of the secreted Dickkopf proteins (Dkk 1, 2, and 4) with high affinity to modulate the canonical Wnt signaling pathway that is transduced by the ternary receptor complex composed of Wnt, Frizzled, and the LDL receptor related protein 5/6 (LRP5/6) co-receptor. Recent experiments have shown that KREMEN2 is a regulator of bone remodeling and raise the possibility that antagonizing KREMEN2 might prove beneficial in patients with bone loss disorders.
Background Reference 1:
Nakamura et al. Biochim. Biophys. Acta. 2001; 1518:63-72.
Background Reference 2:
Mao et al. Nature 2002; 417:664-7.
Background Reference 3:
Li et al. Fertil. Steril. 2008; 90:1470-9.
Background Reference 4:
Schulze et al. PLoS 2010; 5:e10309.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7263

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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