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Western blot analysis of MTERFD1 in 3T3 cell lysate with MTERFD1 antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of MTERFD1 in 3T3 cells with MTERFD1 antibody at 5 μg/mL.
Immunofluorescence of MTERFD1 in 3T3 cells with MTERFD1 antibody at 20 μg/mL.
Western blot analysis of MTERFD1 in 3T3 cell lysate with MTERFD1 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

MTERFD1 Antibody

CATALOG NUMBER: 7281

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (88%), Chicken: (88%)
Immunogen:
MTERFD1 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human MTERFD1 .
The immunogen is located within the last 50 amino acids of MTERFD1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
MTERFD1 Antibody can be used for detection of MTERFD1 by Western blot starting at 1 μg/mL. Antibody can also be used for Immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least three isoforms of MTERFD1 are known to exist. MTERFD1 antibody is predicted to not cross-react with other MTERFD protein family members.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2:
Cat. No. 17-201 - 3T3/BALB Cell Slide
Predicted Molecular Weight:
Predicted: 46 kDa
Observed: 42 kDa
Purification:
MTERFD1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MTERFD1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MTERFD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
MTERFD1
Additional Names:
MTERFD1 Antibody: CGI-12, mTERF3, mTERF domain-containing protein 1, mitochondrial, Mitochondrial transcription termination factor 3
Protein Accession Number:
NP_057026
Protein Gi Number:
34147676
Ncbi Gene Id Number:
51001
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MTERFD1 Antibody: Members of the mTERF (mitochondrial transcription termination factor) family, are mitochondrial proteins that are believed to be transcription termination factors. MTERFD1 is composed of three leucine zippers that form a three-stranded coiled-coil that binds to DNA. It binds promoter DNA and acts as a mitochondrial transcription regulator. It has been suggested that only the phosphorylated form of MTERFD1 has transcription termination activity. A closely related mTERF family member, MTERFD3, is believed to be involved in cell cycle regulation and cell growth by modulating mitochondrial transcription.
Background Reference 1:
Fernandez-Silva P, et al. The human mitochondrial transcription termination factor (mTERF) is a multizipper protein but binds to DNA as a monomer, with evidence pointing to intramolecular leucine zipper interactions. EMBO J. 1997; 16:1066-79.
Background Reference 2:
Hyvarinen AK, Pohjoismaki JL, Holt IJ, et al. Overexpression of MTERFD1 or MTERFD3 impairs the completion of mitochondrial DNA replication. Mol. Biol. Rep. 2011; 38:1321-8.
Background Reference 3:
Prieto-Martin A, Montoya J, Martinez-Azorin F, et al. Phosphorylation of rat mitochondrial transcription termination factor (mTERF) is required for transcription termination but not for binding to DNA. Nucleic Acids Res. 2004; 32:2059-68.
Background Reference 4:
Park CB, Asin-Cayuela J, Camara Y, et al. MTERF3 is a negative regulator of mammalian mtDNA transcription. Cell 2007; 130:273-85.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7281

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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