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Western blot analysis of KANK2 in mouse brain tissue lysate with KANK2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of KANK2 in human kidney tissue with KANK2 antibody at 2.5 μg/ml.
Immunofluorescence of KANK2 in human kidney tissue with KANK2 antibody at 20 μg/ml.
Western blot analysis of KANK2 in mouse brain tissue lysate with KANK2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

KANK2 Antibody

CATALOG NUMBER: 7309

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (94%)
Immunogen:
KANK2 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human KANK2 .
The immunogen is located within the last 50 amino acids of KANK2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
KANK2 Antibody can be used for detection of KANK2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Two alternatively spliced transcript variants encoding different isoforms have been identified.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
Predicted: 95 kDa
Observed: 102 kDa
Purification:
KANK2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
KANK2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
KANK2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
KANK2
Additional Names:
KANK2 Antibody: SIP, MXRA3, ANKRD25, KIAA1518, SIP, KN motif and ankyrin repeat domain-containing protein 2, Ankyrin repeat domain-containing protein 25
Protein Accession Number:
NP_056308
Protein Gi Number:
258613875
Ncbi Gene Id Number:
25959
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
KANK2 Antibody: Ankyrins are membrane adaptor molecules that play important roles in the control of cytoskeleton formation by regulating actin polymerization. KANK2 (KN motif and ankyrin repeat domain-containing protein 2), was initially identified as a steroid receptor coactivator (SRC) interacting protein (SIP) that could sequester steroid receptor coactivators in the cytoplasm. More recent experiments have shown that while KANK2 is widely expressed, in kidney podocytes, KANK2 localizes to foot processes, suggesting that KANK2 may contribute to controlling actin dynamics in podocyte foot processes.
Background Reference 1:
Zhu Y, Kakinuma N, Wang Y, et al. Kank proteins: a new family of ankyrin-repeat domain containing proteins. Biochim. Biophys. Acta 2008; 1780:128-33.
Background Reference 2:
Roy BC, Kakinuma N, Kiyama R. Kank attenuates actin remodeling by preventing interaction between IRSp53 and Rac1. J. Cell Biol. 2009; 184:253-67.
Background Reference 3:
Zhang Y, Zhang H, Liang J, et al. SIP, a novel ankyrin repeat containing protein, sequesters steroid receptor coactivators in the cytoplasm. EMBO J. 2007; 26:2645-57.
Background Reference 4:
Xu X, Patrakka J, Sistani L, et al. Expression of novel podocyte-associated proteins sult1b1 and ankrd25. Nephron Exp. Nephrol. 2011; 117:e39-46.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7309

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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