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Western blot analysis of TRIM24 in A431 cell lysate with TRIM24 antibody at (A) 0.5 and (B) 1 μg/mL.
Immunocytochemistry of TRIM24 in EL4 cells with TRIM24 antibody at 2.5 μg/ml.
Immunofluorescence of TRIM24 in EL4 cells with TRIM24 antibody at 20 μg/ml.
Western blot analysis of TRIM24 in A431 cell lysate with TRIM24 antibody at (A) 0.5 and (B) 1 μg/mL.
Trial Size

TRIM24 Antibody

CATALOG NUMBER: 7383

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TRIM24 antibody was raised against a 19 amino acid peptide near the center of human TRIM24.
The immunogen is located within amino acids 530 - 580 of TRIM24.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
TRIM24 antibody can be used for detection of TRIM24 by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of TRIM24 are known to exist.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Positive Control 2:
Cat. No. 17-207 - EL4 Cell Slide
Predicted Molecular Weight:
Predicted: 112, 116
Observed: 108, 1 of 2 isoforms
Purification:
TRIM24 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRIM24 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRIM24 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TRIM24
Additional Names:
TRIM24 Antibody: PTC6, TF1A, TIF1, RNF82, TIF1A, hTIF1, TIF1ALPHA, Transcription intermediary factor 1-alpha, E3 ubiquitin-protein ligase TRIM24, TIF1-alpha
Protein Accession Number:
NP_056989
Protein Gi Number:
47419911
Ncbi Gene Id Number:
8805
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIM24 Antibody: TRIM24 (TIF1), a member of the tripartite motif (TRIM) family, plays a role in the regulation of cell proliferation and apoptosis. TRIM24 localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The TRIM motif includes three zinc-binding domains (RING, B-box type 1 and B-box type 2) and a coiled-coil region. TIF1 mediates transcriptional events by interactions with the AF2 region of several nuclear receptors, such as the estrogen, retinoic acid and vitamin D3 receptors. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC).
Background Reference 1:
Fraser RA, Heard DJ, Adam S, et al. The putative cofactor TIF1 is a protein kinase that is hyperphosphorylated upon interaction with liganded nuclear receptors. J. Biol. Chem. 1998; 273:16199-204.
Background Reference 2:
Tisserand J, Khetchoumian K, Thibault C, et al. Tripartite motif 24 (Trim24/Tif1a) tumor suppressor protein is a novel negative regulator of interferon (IFN)/signal transducers and activators of transcription (STAT) signaling pathway acting through retinoic acid receptor a (Rara) inhibition. J. Biol. Chem. 2011; 286:33369-79.
Background Reference 3:
Herquel B, Ouararhni K, Khetchoumian K, et al. Transcription cofactors TRIM24, TRIM28, and TRIM33 associate to form regulatory complexes that suppress murine hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 2011; 108:8212-7.
Background Reference 4:
Klugbauer S and Rabes HM. The transcription co-activator HTIF1 and a related protein are fused to the RET receptor tyrosine kinase in childhood papillary thyroid carcinomas. Oncogene 1999;18:4388-93.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7383

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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