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Western blot analysis of 20 ng of (A) viral p24 and (B) recombinant p24 with anti-HIV-1 p24 antibody PM-6335 at (A) 0.5 μg/mL and (B) 0.2 μg/mL, respectively.
Titration ELISA of anti-HIV-1 p24 antibody PM-6335 with 100 ng of recombinant HIV-1 p24 protein.
Western blot analysis of 20 ng of (A) viral p24 and (B) recombinant p24 with anti-HIV-1 p24 antibody PM-6335 at (A) 0.5 μg/mL and (B) 0.2 μg/mL, respectively.
Trial Size

HIV-1 p24 Antibody [8G9]

CATALOG NUMBER: PM-6335

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Virus
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Virus
Immunogen:
Mouse monoclonal HIV-1 p24 antibody was raised against a recombinant full-length HIV-1 p24 protein.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PM-6335 can be used for detection of p24 by Western blot or ELISA at 0.2 – 0.5μg/mL.
Specificity:
By Western blot, anti-HIV-1 p24 antibody detects a ~24 kDa, a ~41 kDa, and a ~55 kDa protein, corresponding to HIV-1 p24 and to its precursors p41 and p55, respectively, in HIV-1 samples.
Positive Control 1:
Cat. No. 95-153 - Recombinant HIV-1 p24
Predicted Molecular Weight:
Predicted: 24, 41, 55 kDa
Purification:
HIV-1 p24 Monoclonal Antibody is Protein A purified.
Clonality:
Monoclonal
Isotype:
IgG1
physical-state:
Liquid
Buffer:
HIV-1 p24 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
HIV-1 p24 Monoclonal Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures
Ncbi Official Symbol:
GAG
Additional Names:
HIV-1 p24 Antibody [8G9] :
Protein Accession Number:
AAB50258
Protein Gi Number:
327745
Ncbi Gene Id Number:
155030
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
HIV-1 p24 Monoclonal Antibody: The human immunodeficiency virus type 1 (HIV-1) particle consists of an envelope, a core and the region between the two termed matrix (1). The HIV-1 Gag protein is a late structural protein that contains four proteins: matrix (p17), capsid (p24), nucleocapsid (p7) and the p6 protein (2). The p24 constitutes the major core component of the virus and shows high degree of sequence conservation among HIV isolates. The Gag p24 has been used as an integral part of multicomponent HIV-1 vaccines (3).
Background Reference 1:
Goto T, Nakai M, and Ikuta K. The life-cycle of human immunodeficiency virus type 1. Micron 1998; 29:123-38.
Background Reference 2:
Freed EO. HIV-1 gag proteins: diverse functions in the virus life cycle. Virology 1998; 251:1-15.
Background Reference 3:
Flynn BJ, Kastenmuller K, Wille-Reece U, et al. Immunization with HIV Gag targeted to dendritic cells followed by recombinant New York vaccinia virus induces robust T-cell immunity in nonhuman primates. Proc. Natl. Acad. Sci. USA 2011; 108:7131–6.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

PM-6335

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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