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Western blot analysis of TRIM33 in human liver tissue lysate with TRIM33 antibody at (A) 1 and (B) 2 μg/mL.
Immunohistochemistry of TRIM33 in human liver tissue with TRIM33 antibody at 2.5 μg/ml.
Immunofluorescence of TRIM33 in human liver tissue with TRIM33 antibody at 20 μg/ml.
Western blot analysis of TRIM33 in human liver tissue lysate with TRIM33 antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

TRIM33 Antibody

CATALOG NUMBER: 7385

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
TRIM33 antibody was raised against an 18 amino acid peptide near the center of human TRIM33.
The immunogen is located within amino acids 580 - 630 of TRIM33.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
TRIM33 antibody can be used for detection of TRIM33 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
TRIM33 antibody is human, mouse and rat reactive. At least two isoforms of TRIM33 are known to exist.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Predicted Molecular Weight:
Predicted: 70, 124 kDa
Observed: 70 kDa
Purification:
TRIM33 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRIM33 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRIM33 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TRIM33
Additional Names:
TRIM33 Antibody: ECTO, PTC7, RFG7, TF1G, TIF1G, TIFGAMMA, TIF1GAMMA, KIAA1113, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, Protein Rfg7
Protein Accession Number:
NP_056990
Protein Gi Number:
74027249
Ncbi Gene Id Number:
51592
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TRIM33 Antibody: TRIM24 (TIF1alpha), TRIM28 (TIF1beta), and TRIM33 (TIF1gamma) are three related cofactors belonging to the tripartite motif superfamily that interact with distinct transcription factors (1-2). The structure of TRIM33 is similar to TRIM24 and TRIM28, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain) (3). TRIM33 functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells (3). It interacts with the Smad2/3 transcription factor in hematopoietic, mesenchymal, and epithelial cell types to mediate different transcriptional effects in response to TGF-beta (4). It acts as an E3 ubiquitin-protein ligase and promotes SMAD4 ubiquitination (5).
Background Reference 1:
Ransom DG, Bahary N, Niss K, et al. The zebrafish moonshine gene encodes transcriptional intermediary factor 1gamma, an essential regulator of hematopoiesis. PLoS Biol. 2004; 2:E237.
Background Reference 2:
Herquel B, Ouararhni K, Khetchoumian K, et al. Transcription cofactors TRIM24, TRIM28, and TRIM33 associate to form regulatory complexes that suppress murine hepatocellular carcinoma. Proc. Natl. Acad. Sci. USA 2011; 108:8212-7.
Background Reference 3:
He W, Dorn DC, Erdjument-Bromage H, et al. Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. Cell 2006; 125:929-41.
Background Reference 4:
Dupont S, Inui M, Newfeld SJ. Regulation of TGF-β signal transduction by mono- and deubiquitylation of Smads. FEBS Lett. 2012; 586:1913-20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7385

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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