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Western blot analysis of Bim in K562 cell lysate with Bim EL antibody at (A) 1 and (B) 2 μg/mL.
Immunocytochemistry of BIM EL inK562 cells with BIM EL antibody at 2.5 μg/ml.
Immunofluorescence of BIM EL in K562 cells with BIM EL antibody at 2.5 μg/ml.
Western blot analysis of Bim in K562 cell lysate with Bim EL antibody at (A) 1 and (B) 2 μg/mL.
Trial Size

Bim EL Antibody

CATALOG NUMBER: 7421

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Bim EL antibody was raised against a peptide corresponding to 15 amino acids near the amino terminus of human Bim.
The immunogen is located within amino acids 20 - 70 of Bim EL.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
Bim EL antibody can be used for detection of Bim EL by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Bim EL antibody is human and mouse reactive. Multiple isoforms of Bim are known to exist; this antibody only detects the Bim EL isoform.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Positive Control 2:
Cat. No. 17-004 - K-562 Cell Slide
Predicted Molecular Weight:
Predicted: 22 kDa
Observed: 24 kDa
Purification:
Bim EL Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
Bim EL Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
Bim EL antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
BCL2L11
Additional Names:
Bim EL Antibody: BAM, BIM, BOD, Bcl-2-like protein 11, Bcl2-interacting mediator of cell death, Bcl2-L-11
Protein Accession Number:
NP_619527
Protein Gi Number:
20336315
Ncbi Gene Id Number:
10018
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Bim EL Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim, also known as Bcl-2-like protein 11, is a pro-apoptotic member of this family and interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. Multiple isoforms of Bim are known to exist, with Bim EL being the longest isoform (1,2).
Background Reference 1:
O'Connor L, Strasser A, O'Reilly LA, et al. Bim: a novel member of the Bcl-2 family that promotes apoptosis. EMBO J. 1998; 17:384-395.
Background Reference 2:
Hsu SY, Lin P, and Hsueh AJ BOD (Bcl-2-related ovarian death gene) is an ovarian BH3 domain-containing proapoptotic Bcl-2 protein capable of dimerization with diverse antiapoptotic Bcl-2 members. Mol. Endocrinol. 1998; 12:1432-40.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7421

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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