Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of DDC in human lung tissue lysate with DDC antibody at (A) 0.5 and (B) 1 μg/ml.
Immunohistochemistry of DDC in human lung tissue with DDC antibody at 2.5 μg/mL.
Immunofluorescence of DDC in human lung tissue with DDC antibody at 20 μg/mL.
Western blot analysis of DDC in human lung tissue lysate with DDC antibody at (A) 0.5 and (B) 1 μg/ml.
Trial Size

DDC Antibody

CATALOG NUMBER: 7447

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (92%), Guinea pig: (77%), Pig: (77%)
Immunogen:
DDC antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DDC.
The immunogen is located within amino acids 390 - 440 of DDC .
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
DDC antibody can be used for detection of DDC by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
DDC antibody is human, mouse and rat reactive.
Positive Control 1:
Cat. No. 1302 - Human Lung Tissue Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
Predicted: 53 kDa
Observed: 53 kDa
Purification:
DDC Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
DDC Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
DDC antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
DDC
Additional Names:
DDC Antibody : AADC, AADC, Aromatic-L-amino-acid decarboxylase, DOPA decarboxylase
Protein Accession Number:
NP_000781
Protein Gi Number:
4503281
Ncbi Gene Id Number:
1644
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
DOPA decarboxylase (DDC) belongs to the group II decarboxylase family of proteins (1). It is an important protein in the catecholamine biosynthesis pathway. DDC catalyzes the second reaction in the biosynthesis of catecholamines, trace amines and serotonin (1,2). It can form a homodimer and is expressed in the central nervous system (2). DDC can be used as markers for dopaminergic, noradrenergic and serotonergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease, neuroendocrine tumors and drug abuse (3,4). Defects in DDC gene may cause the autosomal recessive disorder AADC deficiency (4).
Background Reference 1:
Berry MD, Juorio AV, Li XM, et al. Aromatic L-amino acid decarboxylase: a neglected and misunderstood enzyme. Neurochem. Res. 1996; 21:1075-87.
Background Reference 2:
Sumi-Ichinose C, Ichinose H, Takahashi E, et al. Molecular cloning of genomic DNA and chromosomal assignment of the gene for human aromatic L-amino acid decarboxylase, the enzyme for catecholamine and Serotonin biosynthesis. Biochemistry 1992; 31:2229-38.
Background Reference 3:
Haycock JW, Becker L, Ang L, et al. Marked disparity between age-related changes in dopamine and other presynaptic dopaminergic markers in human striatum. J. Neurochem. 2003; 87:574-85.
Background Reference 4:
Chang YT, Sharma R, Marsh JL, et al. Levodopa-responsive aromatic L-amino acid decarboxylase deficiency. Ann. Neurol. 2004; 55:435-8.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7447

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369