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Western blot analysis of ERAP1 in SK-N-SH cell lysate with ERAP1 antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of ERAP1 in mouse brain tissue with ERAP1 antibody at 5 μg/mL.
Immunofluorescence of ERAP1 in mouse brain tissue with ERAP1 antibody at 20 μg/mL.
Western blot analysis of ERAP1 in SK-N-SH cell lysate with ERAP1 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

ERAP1 Antibody

CATALOG NUMBER: 7467

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (78%)
Immunogen:
ERAP1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human ERAP.
The immunogen is located within amino acids 730 - 780 of ERAP1 .
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ERAP1 antibody can be used for detection of ERAP1 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
ERAP1 antibody is human and mouse reactive. At least two isoforms of ERAP1 are known to exist; this antibody will detect both isoforms. ERAP1 antibody is predicted to not cross-react with ERAP2.
Positive Control 1:
Cat. No. 1220 - SK-N-SH Cell Lysate
Predicted Molecular Weight:
Predicted: 104 kDa
Observed: 105kDa
Purification:
ERAP1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ERAP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ERAP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ERAP1
Additional Names:
ERAP1 Antibody : ALAP, A-LAP, ARTS1, ERAAP, APPILS, ARTS-1, ERAAP1, PILSAP, PILS-AP, KIAA0525, UNQ584/PRO1154, Endoplasmic reticulum aminopeptidase 1
Protein Accession Number:
NP_057526
Protein Gi Number:
94818901
Ncbi Gene Id Number:
51752
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The endoplasmic reticulum (ER) aminopeptidase 1 (ERAP1), a member of the peptidase M1 family, plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides (1,2). It is also designated as adipocyte-derived leucine aminopeptidase (A-LAP), puromycin-insensitive leucine-specific aminopeptidase (PILS-AP) and aminopeptidase regulator of TNFR1 shedding (ARTS-1) (3). ERAP1 is localized to the lumen of the ER and induced by interferon. It may be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (3,4).
Background Reference 1:
Hattori A, Kitatani K, Matsumoto H, et al. Characterization of recombinant human adipocytederived leucine aminopeptidase expressed in Chinese hamster ovary cells. J. Biochem. 2000; 128:755-62.
Background Reference 2:
Saric T, Chang SC, Hattori A, et al. An IFN-gamma induced aminopeptidase in the ER, ERAP1, trims precursors to MHC class I-presented peptides. Nat. Immunol. 2002; 3:1169-76.
Background Reference 3:
Cui X, Hawari F, Alsaaty S, et al. Identification of ARTS-1 as a novel TNFR1-binding protein that promotes TNFR1 ectodomain shedding. J. Clin. Invest. 2002; 110:515-26.
Background Reference 4:
Akada T, Yamazaki T, Miyashita H, et al. Puromycin insensitive leucyl-specific aminopeptidase (PILSAP) is involved in the activation of endothelial integrins. J. Cell Physiol. 2002; 193: 253-62.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7467

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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