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Western blot analysis of BTLA in Jurkat cell lysate at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of BTLA in human small intestine tissue with BTLA antibody at 2.5 μg/mL.
Immunofluorescence of BTLA in human small intestine tissue with BTLA antibody at 20 μg/mL.
Western blot analysis of BTLA in Jurkat cell lysate at (A) 1 and (B) 2 μg/ml.
Trial Size

BTLA Antibody

CATALOG NUMBER: 7473

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
BTLA antibody was raised against a 17 amino acid peptide near the center of human BTLA.
The immunogen is located within amino acids 170 - 220 of BTLA .
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
BTLA antibody can be used for detection of BTLA by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
BTLA antibody is human specific. At least two isoforms of BTLA are known to exist.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 11-801 - Human Small Intestine Tissue Slide
Predicted Molecular Weight:
Predicted: 32 kDa
Observed: 40kDa
Purification:
BTLA Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
BTLA Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
BTLA antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
BTLA
Additional Names:
BTLA Antibody : BTLA1, CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein
Protein Accession Number:
NP_861445
Protein Gi Number:
145580621
Ncbi Gene Id Number:
151888
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The B- and T-lymphocyte attenuator (BTLA) protein is a member of the immunoglobulin superfamily containing a single immunoglobulin (Ig) domain (1). Like other coihibitory receptors such as CTLA-4 and PD-1, BTLA functions as a receptor that relays inhibitory signals to suppress the immune response, and can inhibit LPS-induced endotoxic shock by suppressing TLR4 signaling in innate immune cells (1,2). Polymorphisms in this gene have also been associated with an increased risk of rheumatoid arthritis (3).
Background Reference 1:
Carreno BM and Collins M. BTLA: a new inhibitory receptor with a B7-like ligand. Trends Immunol. 2003; 24:524-7.
Background Reference 2:
Kobayashi Y, Iwata A, Suzuki K, et al. B and T lymphocyte attenuator inhibits LPS-induced endotoxic shock by suppressing Toll-like receptor 4 signaling in innate immune cells. Proc. Natl. Acad. Sci. USA 2013; 110:5121-6.
Background Reference 3:
Lin SC, Kuo CC, and Chan CH. Association of a BTLA gene polymorphism with a risk of rheumatoid arthritis. J. Biomed. Sci. 2006; 13:853-60.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7473

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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