Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of TBX21 in 293 cell lysate with TBX21 antibody at 1 μg/ml.
Immunofluorescence of TBX21 in 293 cells with TBX21 antibody at 5 μg/mL.
Western blot analysis of TBX21 in 293 cell lysate with TBX21 antibody at 1 μg/ml.
Trial Size

TBX21 Antibody

CATALOG NUMBER: 7477

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
TBX21 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TBX2.
The immunogen is located within the last 50 amino acids of TBX21 .
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
TBX21 antibody can be used for detection of TBX21 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
TBX21 antibody is human and mouse reactive.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Positive Control 2:
Cat. No. 17-010 - HEK293 Cell Slide
Predicted Molecular Weight:
Predicted: 59 kDa
Observed: 55kDa
Purification:
TBX21 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TBX21 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TBX21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TBX21
Additional Names:
TBX21 Antibody : TBET, T-PET, T-bet, TBLYM, TBET, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet, T-box protein 21
Protein Accession Number:
NP_037483
Protein Gi Number:
7019549
Ncbi Gene Id Number:
30009
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box (1,2). Members of this family include transcription factors involved in the regulation of developmental processes (2). Studies in mouse and humans show that TBX21 is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (1,3). Expression of the human ortholog also correlates with interferon-gamma expression in Th1 and natural killer cells, suggesting a TBX21 may play a role in initiating Th1 lineage development from naive Th precursor cells. (3).
Background Reference 1:
Szabo SJ, Kim ST, Costa GL, et al. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell 2000; 100:655-69.
Background Reference 2:
Naiche LA, Harrelson Z, Kelly RG, et al. T-box genes in vertebrate development. Annu. Rev. Genet. 2005; 39:219-39.
Background Reference 3:
Hibbert L, Pflanz S, De Waal Malefyt R, et al. IL-27 and IFN-alpha signal via Stat1 and Stat3 and induce T-Bet and IL-12Rbeta2 in naive T cells. J. Interfeon Cytokine Res. 2003; 23:513-22.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7477

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369