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Western blot analysis of IL-28B in HeLa cell lysate with IL-28B antibody at (A) 1 and (B) 2 μg/ml.
Immunofluorescence of IL-28B in HeLa cells with IL-28B antibody at 5 μg/mL.
Western blot analysis of IL-28B in HeLa cell lysate with IL-28B antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

IL-28B Antibody

CATALOG NUMBER: 7479

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
IL-28B antibody was raised against a 17 amino acid peptide near the center of human IL-28B.
The immunogen is located within amino acids 120 - 170 of IL-28B .
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
IL-28B antibody can be used for detection of IL-28B by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
IL-28B antibody is human specific. IL-28B antibody may cross-react with IL-28A and IL-29.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Positive Control 2:
Cat. No. 17-001 - HeLa Cell Slide
Predicted Molecular Weight:
Predicted: 20 kDa
Observed: 16kDa
Purification:
IL-28B Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
IL-28B Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
IL-28B antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
IFNL3
Additional Names:
IL-28B Antibody : IL28B, IL28C, IL-28B, IL28B, ZCYTO22, Interferon lambda-3, Cytokine Zcyto22, IFN-lambda-3
Protein Accession Number:
NP_742151
Protein Gi Number:
28144901
Ncbi Gene Id Number:
282617
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Interleukin 28 (IL-28B), also known as interferon lambda 3, is cytokine distantly related to type I interferons and the IL-10 family (1). The IL-28B gene and the closely related cytokine genes IL-28A and IL-29 form a cytokine gene cluster on a chromosomal region mapped to 19q13. Expression of the cytokines encoded by the three genes can be induced by viral infection and can induce anti-viral activity (2,3). All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor-beta (IL10RB) and interleukin 28 receptor- alpha (IL28RA) (1).
Background Reference 1:
Sheppard P, Kindsvogel W, Xu W, et al. IL-28, IL-29 and their class II cytokine receptor IL-28R. Nat. Immunol. 2003; 4:63-8.
Background Reference 2:
Osterlund PI, Pietila TE, Veckman V, et al. IFN regulatory factor family members differentially regulate the expression of type III IFN (IFN-lambda) genes. J. Immunol. 2007; 179:3434-42.
Background Reference 3:
Meager A, Visvalingam K, Dilger P, et al. Biological activity of interleukins-28 and -29: comparison with type I interferons. Cytokine 2005; 31:109-18.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7479

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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