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Western blot analysis of AQP2 in A431 cell lysate with AQP2 antibody at 1 μg/ml.
Immunohistochemistry of AQP2 in human kidney tissue with AQP2 antibody at 5 μg/mL.
Immunofluorescence of AQP2 in human kidney tissue with AQP2 antibody at 20 μg/mL.
Western blot analysis of AQP2 in A431 cell lysate with AQP2 antibody at 1 μg/ml.
Trial Size

AQP2 Antibody

CATALOG NUMBER: 7621

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Sheep: (100%), Bovine: (95%)
Immunogen:
AQP2 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human AQP2.
The immunogen is located within the last 50 amino acids of AQP2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
AQP2 antibody can be used for detection of AQP2 by Western blot at 1 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
AQP2 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with other members of the aquaporin protein family.
Positive Control 1:
Cat. No. 1202 - A431 Cell Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
Predicted: 30 kDa
Observed: 28 kDa
Purification:
AQP2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
AQP2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
AQP2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
AQP2
Additional Names:
AQP2 Antibody: AQP-CD, WCH-CD, Aquaporin-2, ADH water channel, AQP-2
Protein Accession Number:
NP_000477
Protein Gi Number:
4502179
Ncbi Gene Id Number:
359
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Aquaporins are membrane proteins that serve in the transfer of water and small solutes across cellular membranes. One such aquaporin, aquaporin-2 (AQP2) is located in the kidney collecting tubule and plays a critical role in water reabsorbtion (1). AQP2 is mainly localized in intracellular vesicles but upon stimulation with anti-diuretic hormone (ADH), AQP2 is translocated to the apical plasma membrane by exocytic fusion of AQP2-bearing vesicles (2). Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus (3).
Background Reference 1:
Denker BM, Smith BL, Kuhada FP, et al. Identification, purification, and partial characterization of a novel Mr 28,000 integral membrane protein from erythrocytes and renal tubules. J. Biol. Chem. 1988; 263:15634-42.
Background Reference 2:
Barile M, Pisitkun T, Yu MJ, et al. Large scale protein identification in intracellular aquaporin-2 vesicles from renal inner medullary collecting duct. Mol. Cell Proteomics 2005; 4:1095-106.
Background Reference 3:
Bockenhauer D and Bichet DG. Inherited secondary nephrogenic diabetes insipidus: concentrating on humans. Am. J. Physiol. Renal Physiol. 2013; 304:F1037-42.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7621

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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