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Western blot analysis of GLIPR1L2 in human testis tissue lysate with GLIPR1L2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of GLIPR1L2 in human testis tissue with GLIPR1L2 antibody at 5 μg/mL.
Immunofluorescence of GLIPR1L2 in human testis tissue with GLIPR1L2 antibody at 20 μg/mL.
Western blot analysis of GLIPR1L2 in human testis tissue lysate with GLIPR1L2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

GLIPR1L2 Antibody

CATALOG NUMBER: 7639

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
GLIPR1L2 antibody was raised against an 15 amino acid peptide near the center of human GLIPR1L2.
The immunogen is located within amino acids 120 - 170 of GLIPR1L2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GLIPR1L2 antibody can be used for detection of GLIPR1L2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
GLIPR1L2 antibody is human specific. At least two isoforms of GLIPR1L2 are known to exist; this antibody will detect both isoforms. This antibody is predicted to not cross-react with other GLIPR or GLIPR-like proteins.
Positive Control 1:
Cat. No. 1313 - Human Testis Tissue Lysate
Positive Control 2:
Cat. No. 11-701 - Human Testis Tissue Slide
Predicted Molecular Weight:
Predicted: 28, 31, 38 kDa
Observed: 28 kDa
Purification:
GLIPR1L2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GLIPR1L2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GLIPR1L2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
GLIPR1L2
Additional Names:
GLIPR1L2 Antibody: GLIPR1-like protein 2
Protein Accession Number:
NP_001257325
Protein Gi Number:
394025727
Ncbi Gene Id Number:
144321
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The GLIPR1-like 2 protein (GLIPR1L2) gene is part of a p53 target gene cluster that includes the related proteins GLIPR1 and GLIPR1L2 (2). GLIPR1L2 is similar to both the pathogenesis-related protein (PR) superfamily and the cysteine-rich secretory protein (CRISP) family (1). GLIPR1 is a tumor suppressor whose expression is regulated by p53 (3). Deletions of the GLIPR1/GLIPR1L1/GLIPR1L2 gene cluster have been observed in some multiple myeloma samples, suggesting that these proteins may also be involved in the pathogenesis of multiple myeloma (4).
Background Reference 1:
Ren C, Ren CH, Li L, et al. Identification and characterization of RTVP1/GLIPR1-like genes, a novel p53 target gene cluster. Genomics 2006; 88:163-72.
Background Reference 2:
Murphy EV, Zhang Y, Zhu W, et al. The human glioma pathogenesis-related protein is structurally related to pathogenesis-related proteins and its gene is expressed specifically in brain tumors. Gene 1995; 159:131-5.
Background Reference 3:
Ren C, Li L, Yang G, et al. RTVP-1, a tumor suppressor inactivated by methylation in prostate cancer. Cancer Res. 2004; 64:969-76.
Background Reference 4:
Tam M, Lin P, Hu P, et al. Examining hedgehog pathway genes GLI3, SHH, and PTCH1 and the p53 target GLIPR1/GLIPR1L1/GLIPR1L2 gene cluster using fluorescence in situ hibridization uncovers GLIPR1/GLIPR1L1/GLIPR1L2 deletion in 9% of patients with multiple myeloma. J. Assoc. Genet. Technol. 2010; 36:111-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7639

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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