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Western blot analysis of ARR3 in EL4 cell lysate with ARR3 antibody at (A) 0.5 and (B) 1 μg/ml.
Immunohistochemistry of ARR3 in mouse spleen tissue with ARR3 antibody at 2 μg/ml.
Immunofluorescence of ARR3 in mouse spleen tissue with ARR3 antibody at 20 μg/ml.<br><br>Green: ARR3 Antibody (7643) <br> Blue: DAPI staining
Western blot analysis of ARR3 in EL4 cell lysate with ARR3 antibody at (A) 0.5 and (B) 1 μg/ml.
Trial Size

ARR3 Antibody

CATALOG NUMBER: 7643

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ARR3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human ARR3.
The immunogen is located within the last 50 amino acids of ARR3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ARR3 antibody can be used for detection of ARR3 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
ARR3 antibody is human, mouse and rat reactive. At least three isoforms of ARR3 are known to exist. This antibody is predicted to not cross-react with other members of the arrestin protein family.
Positive Control 1:
Cat. No. 1287 - EL4 Cell Lysate
Predicted Molecular Weight:
Predicted: 43 kDa
Observed: 47 kDa
Purification:
ARR3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ARR3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ARR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ARR3
Additional Names:
ARR3 Antibody: ARRX, ARRX, CAR, Arrestin-C, Cone arrestin, C-arrestin
Protein Accession Number:
NP_004303
Protein Gi Number:
156071467
Ncbi Gene Id Number:
407
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Arrestin 3 (ARR3) belongs to the Arrestin family of proteins that function as negative regulators of G protein-coupled receptor (GPCR) signaling (1,2). ARR3 binds the c-Jun N-terminal kinase 3 (JNK3) and scaffolds the apoptosis signal-regulating kinase 1 (ASK1)-MAPK kinase4-JNK3 cascade in a receptor-independent fashion, promoting JNK3 phosphorylation (3,4). ARR3 also mediates the internalization of the C-C chemokine receptor CCR7 upon the binding of its ligand CCL19, but not CCL21, suggesting that it helps mediate CCR7 receptor desensitization (5).
Background Reference 1:
Murakami A, Yajima T, Sakuma H, et al. X-arrestin: a new retinal arrestin mapping to the X chromosome. FEBS Lett. 1993; 334:203-9.
Background Reference 2:
Gurevich VV and Gurevich EV. The structural basis of arrestin-mediated regulation of G-protein-coupled receptors. Pharmacol. Ther. 2006; 110:465-502.
Background Reference 3:
Song X, Coffa S, Fu H, et al. How does arrestin assemble MAPKs into a signaling complex? J. Biol. Chem. 2009; 284:685-95.
Background Reference 4:
Seo J, Tsakem EL, Breitman M, et al. Identification of arrestin-3-specific residues necessary for JNK3 kinase activation. J. Biol. Chem. 2011; 286:27894-901.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7643

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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