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Western blot analysis of ITCH in 3T3 cell lysate with ITCH antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Western blot analysis of ITCH in 3T3 cell lysate with ITCH antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ITCH Antibody

CATALOG NUMBER: 7645

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ITCH antibody was raised against a 15 amino acid peptide near the amino terminus of human ITCH .
The immunogen is located within amino acids 40 - 90 of ITCH.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ITCH antibody can be used for detection of ITCH by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.
Specificity:
ITCH antibody is human, mouse and rat reactive. At least three isoforms of ITCH are known to exist; this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.
Positive Control 1:
Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Predicted Molecular Weight:
Predicted: 99 kDa
Observed: 99 kDa
Purification:
ITCH antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
ITCH antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ITCH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
ITCH
Additional Names:
ITCH Antibody: AIF4, AIP4, NAPP1, dJ468O1.1, E3 ubiquitin-protein ligase Itchy homolog, Atrophin-1-interacting protein 4, Itch
Protein Accession Number:
NP_001244066
Protein Gi Number:
380420335
Ncbi Gene Id Number:
83737
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The Itchy E3 ubiquitin protein ligase (ITCH) is a member of the Nedd4 family of HECT domain E3 ubiquitin ligases (1). HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. ITCH plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses (2). In B cells, ITCH is thought to associate with latent membrane protein 2A (LMP2A) of Epstein-Barr virus, specifically down-regulating its activity in B cell signaling (3). Mutations in this gene are a cause of syndromic multisystem autoimmune disease (4).
Background Reference 1:
Perry WL, Hustad CM, Swing DA, et al. The itchy locus encodes a novel ubiquitin protein ligase that is disrupted in a18H mice. Nat. Genet. 1998; 18:143-6.
Background Reference 2:
Melino G, Gallagher E, Aqeilan RI, et al. Itch: a HECT-type E3 ligase regulating immunity, skin and cancer. Cell Death Differ. 2008; 15:1103-12.
Background Reference 3:
Ikeda A, Caldwell RG, Longnecker R, et al. Itchy, a Nedd4 ubiquitin ligase, downregulates latent membrane protein 2A activity in B-cell signaling. J. Virol. 2003; 77:5529-34.
Background Reference 4:
Matesic LE, Copeland NG, and Jenkins NA. Itchy mice: the identification of a new pathway for the development of autoimmunity. Curr. Top. Microbiol. Immunol. 2008; 321:185-200.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7645

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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