Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of CXCR3 in HepG2 lysate with CXCR3 antibody at (A) 1 and (B) 2 μg/ml.
Immunocytochemistry of CXCR3 in HepG2 cells with CXCR3 antibody at 5 μg/mL.
Immunofluorescence of CXCR3 in HepG2 cells with CXCR3 antibody at 20 μg/mL.
Western blot analysis of CXCR3 in HepG2 lysate with CXCR3 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

CXCR3 Antibody

CATALOG NUMBER: 7647

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Goat: (89%), Bovine: (88%)
Immunogen:
CXCR3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human CXCR3 .
The immunogen is located within the last 50 amino acids of CXCR3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
CXCR3 antibody can be used for detection of CXCR3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
CXCR3 antibody is human, mouse and rat reactive. At least two isoforms of CXCR3 are known to exist; this antibody will detect both isoforms.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Positive Control 2:
Cat. No. 17-011 - Hep G2 Cell Slide
Predicted Molecular Weight:
Predicted: 46 kDa
Observed: 43 kDa
Purification:
CXCR3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
CXCR3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
CXCR3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
CXCR3
Additional Names:
CXCR3 Antibody: GPR9, MigR, CD182, CD183, Mig-R, CKR-L2, CMKAR3, IP10-R, GPR9, C-X-C chemokine receptor type 3, CXC-R3
Protein Accession Number:
NP_001136269
Protein Gi Number:
218563730
Ncbi Gene Id Number:
2833
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The CXCR3 protein is a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-gamma), CXCL10/IP10 (interferon-gamma-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell alpha-chemoattractant) (reviewed in 1). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration (2). Similar to other chemokine receptors such as CXCR4, CXCR3 has been implicated in several malignancies as a biomarker of tumor behavior as well as a potential therapeutic target (3).
Background Reference 1:
Groom JR and Luster AD. CXCR3 ligands: redundant, collaborative and antagonistic functions. Immunol. Cell Biol. 2011; 89:207-15.
Background Reference 2:
Maghazachi AA. Role of chemokines in the biology of natural killer cells. Curr. Top Microbiol. Immunol. 2010; 341:37-58.
Background Reference 3:
Fulton AM. The chemokine receptors CXCR4 and CXCR3 in cancer. Curr. Oncol. Rep. 2009; 11:125-31.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7647

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369