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<strong> Figure 1 WB Validation in Human Cells </strong><br>Loading: 15 μg of lysate Antibodies: TRPV4, 7695, 0.5 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 WB Validation in Human Tissues </strong><br>Loading: 15 μg of lysate Antibodies: TRPV4, 7695, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 WB Validation in Mouse Tissues </strong><br>Loading: 15 μg of lysate Antibodies: TRPV4, 7695, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 WB Validation in Rat Tissues </strong><br>Loading: 15 μg of lysate Antibodies: TRPV4, 7695, 0.5 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 5 Immunofluorescence Validation of TRPV4 in Human Kidney</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed human kidney tissue labeling TRPV4 with 7695 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red) and DAPI staining (blue).
<strong>Figure 6 Immunohistochemistry Validation of TRPV4 in Human Liver</strong><br> Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-TRPV4 antibody (7695) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of TRPV4 in Mouse Kidney</strong><br> Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRPV4 antibody (7695) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunohistochemistry Validation of TRPV4 in Rat Kidney</strong><br> Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-TRPV4 antibody (7695) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Figure 1 WB Validation in Human Cells
Loading: 15 μg of lysate Antibodies: TRPV4, 7695, 0.5 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

TRPV4 Antibody

CATALOG NUMBER: 7695

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%), Mouse: (100%)
Immunogen:
Anti-TRPV4 antibody (7695) was raised against a peptide corresponding to 18 amino acid synthetic peptide near the center of human TRPV4.

The immunogen is located within amino acids 380-430 of TRPV4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Application Note:
WB: 0.5-2 μg/mL; IHC-P: 1-2 μg/mL; IHC-P/IF: 5-20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunofluorescence in the human sample. All other applications and species not yet tested.
Specificity:
TRPV4 antibody is human specific. At least six isoforms of TRPV4 are known to exist. This antibody is predicted to not cross-react with other TRP protein family members.
Positive Control 1:
Cat. No. 1402 - Mouse Lung Tissue Lysate
Positive Control 2:
Cat. No. 1465 - Rat Kidney Tissue Lysate
Predicted Molecular Weight:
Predicted: 98kD

Observed: 98 kD
Purification:
TRPV4 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
TRPV4 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
TRPV4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
TRPV4
Additional Names:
TRPV4 Antibody: SMAL, VRL2, CMT2C, SPSMA, TRP12, VROAC, HMSN2C, OTRPC4, SSQTL1, Transient receptor potential cation channel subfamily V member 4, Osm-9-like TRP channel 4, TrpV4
Protein Accession Number:
NP_067638
Protein Gi Number:
22547184
Ncbi Gene Id Number:
59341
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The transient receptor potential (TRP) protein family consists of a diverse group of cation channels functioning in a variety of homeostatic and regulatory pathways. Four subfamilies exist, based on channel domain homology: C type (canonical), V type (vanilloid receptor related), M type (melastatin related) and P type (PKD) (1). TRPV4, belongs to the V type subfamily and plays a role in systemic osmoregulation (2,3). TRPV4 is a calcium channel multi-pass membrane protein activated by various stimuli, including thermal stress, fatty acid metabolites and hypotonicity (3). TRPV4 is highly expressed in lung and kidney and widely expressed in brain. It plays an important role in regulating neural excitability (4).
Background Reference 1:
Birnbaumer L, Yildirim E and Abramowitz J. A comparison of the genes coding for canonical TRP channels and their M, V and P relatives. Cell Calcium 2003; 33:419-32.
Background Reference 2:
Alessandri-Haber N, Dina OA, Yeh JJ, et al. Transient receptor potential vanilloid 4 is essential in chemotherapy-induced neuropathic pain in the rat. J. Neurosci. 2004; 24:4444-52.
Background Reference 3:
Liedtke W. TRPV4 plays an evolutionary conserved role in the transduction of osmotic and mechanical stimuli in live animals. J. Physiol. 2005; 567:53-8.
Background Reference 4:
Shibasaki K, Suzuki M, Mizuno A, et al. Effects of body temperature on neural activity in the hippocampus: regulation of resting membrane potentials by transient receptor potential vanilloid 4. J. Neurosci. 2007; 27:1566–75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7695

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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