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Western blot analysis of GDPD5 in mouse kidney tissue lysate with GDPD5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of GDPD5 in human kidney tissue with GDPD5 antibody at 5 μg/mL.
Immunofluorescence of GDPD5 in human kidney tissue with GDPD5 antibody at 20 μg/mL.
Western blot analysis of GDPD5 in mouse kidney tissue lysate with GDPD5 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

GDPD5 Antibody

CATALOG NUMBER: 7739

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Chicken: (94%)
Immunogen:
GDPD5 antibody was raised against an 18 amino acid peptide near the amino terminus of human GDPD5.
The immunogen is located within the first 50 amino acids of GDPD5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
GDPD5 antibody can be used for detection of GDPD5 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
GDPD5 antibody is human, mouse and rat reactive. At least three isoforms of GDPD5 are known to exist.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Positive Control 2:
Cat. No. 10-401 - Human Kidney Tissue Slide
Predicted Molecular Weight:
Predicted: 40, 67 kDa
Observed: 52 kDa
Purification:
GDPD5 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
GDPD5 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
GDPD5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
GDPD5
Additional Names:
GDPD5 Antibody: GDE2, PP1665, GDE2, PP6037, PP9363, UNQ1850/PRO3580, Glycerophosphodiester phosphodiesterase domain-containing protein 5, Glycerophosphodiester phosphodiesterase 2
Protein Accession Number:
NP_110419
Protein Gi Number:
189571657
Ncbi Gene Id Number:
81544
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) protein, also known as GDE2, is a seven transmembrane, widely expressed protein (1) that is necessary for spinal motor neuron differentiation and retinoid-induced neuronal outgrowth (2,3). Altered choline phospholipid metabolism is a hallmark of cancer, and the elevated expression of GDPD5 correlates with malignant choline phospholipid metabolite profiles in human breast cancer (4).
Background Reference 1:
Nogusa Y, Fujioka Y, Komatsu R, et al. Isolation and characterization of two serpentine membrane proteins containing glycerophosphodiester phosphodiesterase, GDE2 and GDE6. Gene 2004; 337:173-9.
Background Reference 2:
Rao M and Sockanathan S. Transmembrane protein GDE2 induces motor neuron differentiation in vivo. Science 2005; 309:2212-5.
Background Reference 3:
Yanaka N, Nogusa Y, Fujioka Y, et al. Involvement of membrane protein GDE2 in retinoic acid-induced neurite formation in Neuro2A cells. FEBS Lett. 2007; 581:712-8.
Background Reference 4:
Cao MD, Dopkens M, Krishnamachary B, et al. Glycerophosphodiester phosphodiesterase domain containing 5 (GDPD5) expression correlates with malignant choline phospholipid metabolite profiles in human breast cancer. NMR Biomed. 2012; 25:1033-42.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7739

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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