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Western blot analysis of SREBF2 in PC-3 cell lysate with SREBF1 antibody at 1 μg/ml.
Immunohistochemistry of SREBF2 in human spleen tissue with SREBF2 antibody at 5 μg/mL.
Immunofluorescence of SREBF2 in human spleen tissue with SREBF2 antibody at 20 μg/mL.
Western blot analysis of SREBF2 in PC-3 cell lysate with SREBF1 antibody at 1 μg/ml.
Trial Size

SREBF2 Antibody

CATALOG NUMBER: 7855

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat: (100%)
Immunogen:
SREBF2 antibody was raised against a 15 amino acid peptide near the center of human SREBF2.
The immunogen is located within amino acids 440 - 490 of SREBF2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
SREBF2 antibody can be used for detection of SREBF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
SREBF2 antibody is human and mouse reactive. At least three isoforms of SREBF2 are known to exist. SREBF2 antibody is predicted not to cross-react with SREBF1.
Positive Control 1:
Cat. No. 1216 - PC-3 Cell Lysate
Positive Control 2:
Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight:
Predicted: 126 kDa
Observed: 128 kDa
Purification:
SREBF2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
SREBF2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
SREBF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
SREBF2
Additional Names:
SREBF2 Antibody: SREBP2, bHLHd2, SREBP-2, BHLHD2, SREBP2, Sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2
Protein Accession Number:
NP_004590
Protein Gi Number:
27477113
Ncbi Gene Id Number:
6721
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The sterol regulatory element binding transcription factor 2 (SREBF2) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1,2). The related protein SREBF1 also binds SRE1 and activates transcription in an additive fashion to SREBF2 (2). SREBF2 has been more closely associated with cholesterol synthesis and accumulation, while the SREBF1 proteins are important in the regulation of genes involved in lipid metabolism (3).
Background Reference 1:
Hua X, Yokoyama C, Wu J, et al. SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc. Natl. Acad. Sci. USA 1993; 90:11603-7.
Background Reference 2:
Wang X, Briggs MR, Hua X, et al. Nuclear protein that binds sterol regulatory element of low density lipoprotein receptor promoter. II. Purification and characterization. J. Biol. Chem. 1993; 268:14497-504.
Background Reference 3:
Raghow R, Yellaturu C, Deng X, et al. SREBPs: the crossroads of physiological and pathological homeostasis. Endocrinol. Metab. 2008; 19:65-73.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7855

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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