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Western blot analysis of MFN2 in human brain tissue lysate with MFN2 antibody at 1 μg/ml.
Immunohistochemistry of MFN2 in rat brain tissue with MFN2 antibody at 5 μg/mL.
Immunofluorescence of MFN2 in rat brain tissue with MFN2 antibody at 20 μg/mL.
Immunofluorescence of MFN2 in mouse brain tissue with MFN2 antibody at 20 μg/mL. <br><br>Green: MFN2 antibody (7863) <br> Red: Phylloidin staining<br> Blue: DAPI staining
Immunohistochemistry of MFN2 in mouse brain tissue with MFN2 antibody at 5 μg/mL.
Western blot analysis of MFN2 in human brain tissue lysate with MFN2 antibody at 1 μg/ml.
Trial Size

MFN2 Antibody

CATALOG NUMBER: 7863

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
MFN2 antibody was raised against a 17 amino acid peptide near the center of human MFN2.
The immunogen is located within amino acids 250 - 300 of MFN2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
MFN2 antibody can be used for detection of MFN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in mouse and rat samples. All other applications and species not yet tested.
Specificity:
MFN2 antibody is human, mouse and rat reactive. At least three isoforms of MFN2 are known to exist. MFN2 antibody is predicted to not cross-react with MFN1.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Predicted Molecular Weight:
Predicted: 83 kDa
Observed: 90 kDa
Purification:
MFN2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
MFN2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
MFN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
MFN2
Additional Names:
MFN2 Antibody: HSG, MARF, CMT2A, CPRP1, CMT2A2, KIAA0214, Mitofusin-2, Transmembrane GTPase MFN2
Protein Accession Number:
NP_055689
Protein Gi Number:
7662004
Ncbi Gene Id Number:
9927
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Mitofusin 2 (MFN2) and the related protein MFN1 are mitochondrial membrane GTPase proteins that play a central role in mitochondrial metabolism and may be associated with obesity and/or apoptosis processes (1,2). MFN2 is ubiquitously expressed, and found in both the ER and outer mitochondrial membrane. MFN2 has two key domains: a coiled coil region that mediates MFN2 binding and a GTPase domain that likely plays a role in fusion (3,4). Both domains are essential for embryonic development and may play a role in the pathobiology of obesity. Overexpression of MFN2 causes mitochondrial dysfunction and cell death (5).
Background Reference 1:
Chen H, Detmer SA, Ewald AJ, et al. Mitofusins Mfn1 and Mfn2 coordinately regulate mitochondrial fusion and are essential for embryonic development. J. Cell Biol. 2003; 160:189-200.
Background Reference 2:
Ishihara N, Eura Y, and Mihara K. Mitofusin 1 and 2 play distinct roles in mitochondrial fusion reactions via GTPase activity. J. Cell Sci. 2004; 117:6535-46.
Background Reference 3:
Bach D, Pich S, Soriano FX, et al. Mitofusin-2 determines mitochondrial network architecture and mitochondrial metabolism. A novel regulatory mechanism altered in obesity. J. Biol. Chem. 2003; 278:17190-7.
Background Reference 4:
Renaldo F, Amati-Bonneau P, Slama A, et al. MFN2, a new gene responsible for mitochondrial DNA depletion. Brain 2012; 135:e223, 1-4.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7863

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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