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Western blot analysis of NDST2 in A-20 cell lysate with NDST2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Immunofluorescence of NDST2 in Daudi cells with NDST2 antibody at 20 μg/ml.
Western blot analysis of NDST2 in A-20 cell lysate with NDST2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Trial Size

NDST2 Antibody

CATALOG NUMBER: 7869

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Bovine: (100%)
Immunogen:
NDST2 antibody was raised against a 16 amino acid peptide near the amino terminus of human NDST2.
The immunogen is located within amino acids 20 - 70 of NDST2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
NDST2 antibody can be used for detection of PDCL by Western blot at 1 - 2 μg/ml. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
NDST2 antibody is human, mouse and rat reactive. At least two isoforms of NDST2 are known to exist; this antibody will only detect the larger isoform. NDST2 antibody is predicted not to cross-react with other members of the NDST protein family.
Positive Control 1:
Cat. No. 1288 - A20 Cell Lysate
Predicted Molecular Weight:
Predicted: 97 kDa
Observed: 101 kDa
Purification:
NDST2 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
NDST2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
NDST2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
NDST2
Additional Names:
NDST2 Antibody: NST2, HSST2, N-heparan sulfate sulfotransferase 2, NDST-2
Protein Accession Number:
NP_003626
Protein Gi Number:
4505353
Ncbi Gene Id Number:
8509
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2 (NDST2) protein, also known as N-heparan sulfate sulfotransferase 2, and the highly related protein NDST1 have key roles in the sulfation of heparan sulfate proteoglycans, molecules that are present of cell surfaces and the extracellular matrix (1,2). NDST1 and NDST2 are dispensable for mesodermal differentiation into osteoblasts but necessary for induction of adipocytes and neural cells (3).
Background Reference 1:
Humphries DE, Lanciotti J, and Karlinsky JB. cDNA cloning, genomic organization and chromosomal localization of human heparan glucosaminyl N-deacetylase/N-sulphotransferase-2. Biochem. J. 1998; 332:303-7.
Background Reference 2:
Esko JD and Selleck SB. Order out of chaos. Assembly of ligand binding sites in heparan sulfate. Annu. Rev. Biochem. 2002; 71:435-71.
Background Reference 3:
Forsberg M, Holmborn K, Kundu S, et al. Undersulfation of heparan sulfate restricts differentiation potential of mouse embryonic stem cells. J. Biol. Chem. 2012; 287:10853-62.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7869

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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